摘要
为了确定湖北某养猪场育肥猪急性死亡的原因,采用细菌分离培养与镜检、PCR鉴定与分型等方法,并进一步对分离菌株进行了药物敏感性分析和致病性研究,利用病理组织切片技术观察病变。结果表明:分离菌株为猪胸膜肺炎放线杆菌血清5型,对昆明小鼠具有较强的致病性,能导致小鼠呼吸困难和死亡;药物敏感性分析显示病原菌对头孢噻肟、头孢噻呋、头孢拉定、头孢曲松、氟苯尼考等药物高度敏感。
To determine the cause of acute death in fattening swines at an industrial pig farm in Hubei Province, the pathogen was identified by isolating and culture, microscopic examination, PCR assay and bacterium classification methods. Furthermore, the drug sensitive test and path- ogenicity test were carried out on isolated bacterium. Results indicated that the pathogenic bacteria was APP seruvar 5, which has strong patho- genicity to Kunming mice and can cause dyspnea and death in mice. Drug sensitivity analysis showed that the pathogen is highly sensitive to ce- fotaxime, ceftiofur, cephradine, cefatriaxone, florfenicol and other drugs..
基金
公益性行业(农业)科研专项(201303034-9)
湖北省农业科学院竞争性计划项目(2015jzxjh03)
兽医生物技术国家重点实验室开放课题基金项目(SKLVBF 201603)
湖北省技术创新专项重大项目(2016ABA124)
关键词
猪传染性胸膜肺炎
胸膜肺炎放线杆菌
分离鉴定
血清型鉴定
药敏试验
porcine contagious pleuropneumonia
Actinobacillus pleuropnettmoniae
isolation and identification
serotyping identification
drug sen- sitive test
