摘要
研究估计了日本囊对虾基础群体的体长、腹长、体质量与耐高氨氮性状的遗传参数,为制定综合选择指数、选择方法与育种目标提供技术参考。试验引进日本囊对虾台湾群体亲本,以1尾亲虾构建1个家系,共建立63个全同胞家系,每个家系单独培育,密度调整后开展共同环境养殖测试。各家系养殖150d后,统计每个家系生长性状,并分别从家系中随机选取30尾个体,在氨氮质量浓度为68.5mg/L下进行耐高氨氮试验,48h后统计各个家系的存活率。利用一般线性动物混合模型与广义线性模型分析方法分别估计生长和耐高氨氮性状的方差组分和遗传参数。试验结果表明,日本囊对虾幼虾体长(估计值0.79±0.13)、腹长(0.74±0.24)与体质量(0.31±0.25)遗传力为中高等遗传力水平性状;耐高氨氮性状为低遗传力水平性状,估计值为0.13±0.06,48h后家系耐高氨氮性状平均值为(8.84±12.65)%,耐高氨氮性状的变异水平为143.10%。体长、腹长、体质量与耐高氨氮性状的表型相关与遗传相关系数分别为-0.082~0.08和-0.067~0.17,检验结果不显著。研究结果表明,采用复合育种技术对日本囊对虾生长性状与耐高氨氮性状同时进行改良,可以起到加快育种进程的作用。
Genetic parameters were estimated for body length, abdomen length, and body weight, and high ammonia nitrogen tolerance of the Go population of kuruma prawn Marsupenaeus japonicus to provide basic parameters for breeding objectives, comprehensive selection index and selection methods. In the ex- periment, Taiwan population of kuruma prawn broodtocks was introducednd and 63 families were estab- lished from one parent prawn by nurturing separately. The growth traits of each family were calculated separately in 150 d culture, and a high ammonia nitrogen tolerance experiment was carried out in 30 indi- viduals sampled randomly from each family under 68. 5 mg/L concentration in 48 h using a two trait linear animal model and threshold model. The heritability was shown to be 0. 79±0. 13 in body length, 0. 74±0.24 in abdomen length, and 0.31±0.25 in body weight, being in medium and high heritability. The heri- tability of high ammonia nitrogen tolerance was estimated at 0. 13±0.06, in low level heritability, and the whole family had average survival rate of （8. 84±12. 65）%, with the correlation coefficient of --0. 082- 0. 08 for phenotype and --0. 067-0. 17 for genetics. No significant correlation between main growth traits and survival rate was found and combined breeding techniques are suggested for genetic improvement and resistance traits in kuruma shrimp in the future.
出处
《水产科学》
CAS
CSCD
北大核心
2017年第6期700-706,共7页
Fisheries Science
基金
广东省海洋与渔业局良种体系专项([2014]301号)
湛江市科技计划项目(2015A03004)
关键词
日本囊对虾
遗传参数
耐高氨氮
约束最大似然法
Marsupenaeus japonicus
genetic parameter
Maximum Likelihood Method （REML）high ammonia nitrogen tolerance
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