摘要
属于糖基转移酶(GTs)家族1的尿苷二磷酸(UDP)-糖基转移酶(UGT)在转移拟南芥中的糖基的转移和糖基化次级代谢物中起重要作用.重建的系统发育树对这个多基因家族的进化关系和功能提供了新的见解和研究.通过综合分析系统发育谱和重建的系统发育树,我们系统地分析了112个拟南芥UGTs.在我们的工作中,我们检测到五个不同的进化保守模块,大多数UGTs分为同一模块,表明拟南芥UGTs在进化过程中是相当保守的.接下来收集拟南芥UGT的底物,并将它们映射到直向同源组,揭示UGT在结合特异性底物中的进化关系.通过扫描271不同物种,还预测一些未报告的蛋白质与拟南芥UGTs密切相关.
Uridine diphosphate （UDP）-glycosyltransferases （UGTs）, belonging to the family 1 glycosyl- transferases （GTs）, play important roles in transferring glycosyl moieties and glycosylating secondary metabolites in Arabidopsis thaliana. Reconstructed phylogenomic trees can provide novel insights in studying evolutionary relationship and functions of this multigene family. By combining comprehensive phylogenetic profiles and reconstructed phylogenomic trees, we systemically analyzed 112 Arabidopsis UGTs. In total, we detected five distinct evolutionary conserved modules, and most UGTs were divided into the same module indicating a rather conserved evolutionary routine of Arabidopsis UGTs. We next manually collected the substrates of Arabidopsis UGTs and mapped them to orthologous groups, revea- ling evolutionary relationships of UGTs in binding specific substrates. By scanning 271 different species, we also predicted several unreported proteins that were closely related to Arabidopsis UGTs.
作者
方阳
牛毓龙
刘程程
林江依
李旭锋
杨毅
FANG Yang;NIU Yu-Long;LIU Cheng-Cheng;LIN Jiang-Yi;LI Xu-Feng;YANG Yi(Key Lab of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Siehuan University, Chengdu 610064, China;State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;Wu YuZhang Honors College of Siehuan University, Chengdu 610064, China)
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第6期1317-1322,共6页
Journal of Sichuan University(Natural Science Edition)
基金
973计划(2015CB755702)
