摘要
香根草能够有效诱集水稻害虫稻蛀茎夜蛾Sesamia inferens雌成虫在其上产卵,但孵化出的幼虫在香根草上不能完成生活史。为了初步明确香根草对稻蛀茎夜蛾幼虫生理生化水平的影响,本研究分析测定了取食香根草后稻蛀茎夜蛾幼虫体内解毒酶和乙酰胆碱酯酶AChE活性的变化。结果表明:与取食水稻的幼虫相比,取食香根草后幼虫解毒酶谷胱甘肽硫转移酶GSTs和AChE活性显著提高。其他两种解毒酶,羧酸酯酶CarE和细胞色素P450酶活性无显著差异。取食香根草后,4龄幼虫GSTs比活力约为取食水稻的2.1倍;2龄幼虫AChE比活力约为取食水稻的2.9倍。本研究结果表明香根草可能存在某些有毒活性物质或者次生代谢物质,诱导了稻蛀茎夜蛾体内GSTs和AChE的活性,这可能是稻蛀茎夜蛾取食香根草后的应急防御机制之一。此研究结果为深入阐明香根草在生理、生化水平上对稻蛀茎夜蛾幼虫的影响奠定了基础,为开发以香根草为基础的稻蛀茎夜蛾绿色防控新技术提供了理论依据。
The vetiver grass Vetiveria zizanioides could effectively attract female adults of Sesamia inferens to lay eggs,while the offspring larvae could not complete their life cycles after feeding on vetiver.Aimed to preliminarily clear the physiological and biochemical effect of vetiver against the larvae of S.inferens,we determined the activities of the detoxification enzymes and acetylcholinesterase(AChE)of S.inferens larvae after feeding on vetiver and rice.The results showed that,compared with the larvae fed on rice,the activities of detoxification enzyme glutathione S-transferase(GSTs)and AChE were significantly increased in the larvae fed on vetiver.However,there was no significant difference in carboxylesterase(CarE)and cytochrome P450 s activities.The GSTs activity in the fourth-instar larvae and the AChE activity in the second-instar larvae fed on vetiver were 2.1-,2.9-fold higher than those in the larvae fed on rice.These results indicated that vetiver may have some active substances or secondary metabolite substances that induced GSTs and CarE activities in the larvae fed on vetiver,which may be one of the mechanisms for emergency adaptation of S.inferens against vetiver.This study lays the foundation for further elucidating the physiological and biochemical effect of vetiver against the larvae of S.infer-ens,and provides a theoretical basis for the development of sustainable control technology for S.inferens based on the trap plant vetiver in rice ecosystem.
出处
《植物保护》
CAS
CSCD
北大核心
2017年第6期123-126,143,共5页
Plant Protection
基金
国家重点研发计划(2016YFD0200800)
重点实验室培育基地(2010DS700124-ZZ1601)
公益性行业(农业)科研专项(201303017)
关键词
稻蛀茎夜蛾
香根草
解毒酶
乙酰胆碱酯酶
诱杀植物
Sesamia inferens
Vetiveria zizanioides
detoxification enzyme
acetylcholinesterase
trap plant
