摘要
预防性给予巨噬细胞(RAW264.7细胞)不同浓度的生物活性肽Gln-Glu-Pro-Val(QEPV)后,用脂多糖(LPS)刺激细胞,通过检测细胞因子表达和炎性蛋白基因转录,观察生物活性肽QEPV对细胞抵御LPS刺激的调节作用。结果表明,0.1g/L的QEPV有显著的促进细胞增殖作用,0.5g/L浓度以下的QEPV对RAW264.7细胞无增殖抑制作用。0.5g/L QEPV预处理RAW264.7细胞后,用LPS刺激细胞,其IL-6、IL-12等促炎细胞因子的分泌降低,抗炎细胞因子IL-10分泌提前,分泌量增加,COX-2、iNOS基因的转录水平降低,说明乳源性生物活性肽QEPV对RAW264.7细胞抵御LPS刺激起正向调节作用。
Pre-feeding different concentrations of bioactive peptide Gln-Glu-Pro-Val(QEPV)was used to evaluate its regulatory effects on the responds of macrophage(RAW264.7)under lipopolysaccharide(LPS)stimulation.The results show that 0.1g/L QEPV has positive effect on cells reproduction and QEPV below 0.5g/L has no reproduction suppression effect on RAW264.7cells.Pre-feeding 0.5g/L QEPV can suppress RAW264.7cells to produce IL-6/IL-12 and promote to produce IL-10.QEPV can suppress the expression of COX-2/iNOS gene in transcription level.The research shows that the bioactive peptide QEPV has positive regulatory effect on protecting RAW264.7cells from LPS stimulation.
出处
《上海交通大学学报（农业科学版）》
2016年第6期14-20,共7页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
国家自然科学基金项目(31491623)
关键词
生物活性肽
巨噬细胞
脂多糖
炎症
bioactive peptide
RAW264.7
lipopolysaccharide(LPS)
inflammation
