摘要
目的:探讨NPAS3基因表达及多态性在闽南地区正常人群与精神分裂症人群中的差异。方法:随机选择闽南地区30例正常人、30例精神分裂症患者作为研究对象,提取外周血RNA,利用RT-PCR检测NPAS3基因的表达;提取外周血DNA后利用特异性引物对其进行扩增,测序后基因分型。结果:RT-PCR结果表明NPAS3基因表达在2组中无显著性差异(P>0.05),rs10483442位点经基因分型发现只有CC一种基因型,rs207474631位点经基因分型发现也只有CC一种基因型,均未发现其他基因型。结论:NPAS3基因表达以及rs10483442和rs207474631位点在闽南地区正常人群和精神分裂症人群中可能不存在差异。
Objective： To investigate the differences of NPAS3 gene expression and po/morphismbetween normal populationand schizophrenia population in southern region of Fujian. Methods： 30 normal people and 30 patients with schizophrenia in southern region of Fujian were randomly selected as research objects. Their RNAwas extracted from peripheral blood to detect the expression of NPAS3 gene by RT-PCR. Their DNAwas extracted from peripheral blood and amplified by specific sequenced for genotyping. Results： RT-PCRresults showed that NPAS3 gene expression had no significant differencc between the two groups （ P 〉0. 05） ; genotyping results showed only CC genotype was found at rs10483442 site and only CC genotype was found at rs207474631 site. Conclusion： NPAS3 gene expression and rs10483442 and rs207474631 sites may have no differences betweennormal and schizophrenic populations in southern region of Fujian.
出处
《现代临床医学》
2017年第6期425-427,共3页
Journal of Modern Clinical Medicine
基金
福建省自然科学基金项目(2012D048)
