摘要
目的:测定滇重楼中4种重楼皂苷和8种重金属元素的含量,探讨上述2种质量评价指标间的相关性。方法:采用电感耦合等离子体发射光谱法测定滇重楼根茎内铅、镉、铜、铬、钡和锑的含量;原子荧光光谱分析法测定汞、砷的含量;高效液相色谱法测定重楼总皂苷(包括重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ和重楼皂苷Ⅶ)的含量,并采用SPSS 23.0软件对数据进行相关性分析。结果:滇重楼根茎中铅、镉、铜、汞、砷、铬、钡和锑的平均含量分别为20.433、50.020、67.852和280.491μg/kg,1.632、1.982、9.356和15.875 mg/kg;重楼总皂苷含量为0.811%,其中重楼皂苷Ⅰ占总皂苷量的72.4%。相关性分析结果显示,重楼总皂苷的含量与8种重金属的含量无显著相关性,但重楼皂苷Ⅰ的含量与镉的含量呈极显著负相关,与铜、锑的含量呈极显著正相关,与砷的含量呈显著正相关。结论:滇重楼中活性成分的含量与重金属元素的含量有一定相关性,可为建立滇重楼全面质量评价体系提供数据依据。
OBJECTIVE：To determine the content of 4 kinds of polyphyllins and 8 kinds of heavy metals in Paris polyphylla var.yunnanensis, so as to probe into the correlation between the indicators of quality evaluation .METHODS：The content of Pb , Cd, Cu, Cr, Ba and Sb were determined by ICP-AES;the content of Hg and As were determined by AFC;the content of Rhizoma Paridis total saponin （ parissaponin Ⅰ, parissaponin Ⅱ, parissaponin Ⅵ and parissaponin Ⅶ） were determined by HPLC;the data were analyzed by SPSS 23.0 software .RESULTS：The average contents of Pb , Cd, Cu, Hg, As, Cr, Ba and Sb in rhizome of Paris polyphylla var.yunnanensis were 20.433 μg/kg, 50.020 μg/kg, 67.852 μg/kg, 280.491 μg/kg, 1.632 mg/kg, 1.982 mg/kg, 9.356 mg/kg and 15.875 mg/kg respectively.The total amount of polyphyllins was 0.811%, of which 72.4% was parissaponin Ⅰ.The correlation analysis indicated that the content of total amount of polyphyllins was not significantly correlated with the content of 8 kinds of heavy metals, yet there was significant negative correlation between the content of parissaponin Ⅰ and Cd, and significant positive correlation with the content of Cu and Sb , as well as significant positive correlation with the content of As . CONCLUSIONS：There is certain correlation between the content of the active components of Paris polyphylla var. yunnanensis and heavy metal elements , which can provide data support for the development of the comprehensive quality evaluation system .
出处
《中国医院用药评价与分析》
2017年第10期1315-1318,共4页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
广西科学研究与技术开发计划项目(No.桂科能1598025-35)
关键词
滇重楼
重金属
重楼皂苷
质量控制
Paris polyphylla var.yunnanensis
Heavy metal
Polyphyllins
Quality control
