摘要
以北柴胡种子为试材,采用不同配比的水杨酸和壳聚糖混合液进行处理(CK:自来水,T1:水杨酸0.01mmol·L^(-1)+壳聚糖1mg·mL^(-1),T2:水杨酸0.01mmol·L^(-1)+壳聚糖10mg·mL^(-1),T3:水杨酸0.05 mmol·L^(-1)+壳聚糖1 mg·mL^(-1),T4:水杨酸0.05mmol·L^(-1)+壳聚糖10mg·mL^(-1)),北柴胡种子浸种24h后播种,在种子萌发过程中测定其生理指标,以研究不同配比的水杨酸和壳聚糖混合液对北柴胡种子萌发的影响。结果表明:T1~T4处理的发芽率、含水量、体积总体呈上升趋势;可溶性糖含量呈现下降-上升-下降的趋势,第10天和第15天是转折点;CK和T4处理在整个过程蛋白质含量呈下降趋势,而T1、T2、T3处理在种子萌发初期先上升之后呈下降趋势;氨基酸含量总体呈先上升后下降的趋势,但达到最高点的时间不同,T3在第5天最早达到最高点,其它处理在第20天最高,且T4的氨基酸含量高于其它处理。用隶属函数分析对柴胡各项生理指标的变化进行综合评价大小顺序为T4>T3>T2>CK>T1。综上可知,不同配比的混合液浸种对北柴胡种子的萌发作用不同,用配比为水杨酸0.05mmol·L^(-1)+壳聚糖10mg·mL^(-1)浸种效果最好。
The seeds of Bupleurum chinense were used as test materials,in order to select the optimum mixture liquid ratio and improve the seed germination rate of Bupleurum chinense seeds,comparative experiments for different ratio of the mixture fluid of Bupleurum chinense seeds namely T1,0.01 mmol·L^（-1） salicylic acid＋1 mg· mL^（-1） chitosan.T2,0.01 mmol· mL^（-1） salicylic acid＋10 mg·mL^（-1） chitosan,T3,0.05 mmol· L^（-1） salicylic acid ＋ 1 mg · mL^（-1） chitosan,T4,0.05 mmol·mL^（-1） salicylic acid＋10 mg· mL^（-1） chitosan were conducted and physiological and biochemical indexes determined at seed germination after the Bupleurum chinense seeds were soaking for 24 hours.The results showed that germination rate,moisture content and the volume showed overall upward trend,and soluble sugar content showed a downward-rising-downward trend for four treatments.A turning point were fond in the day of 10 th and 15 th.The protein content of CK and T4 treatment were decreased in the whole process,T1,T2,T3 treatment showed a rising trend slightly at early germination stage and then showed a downward trend.But the point of time reached its highest of amino acid content was different.T3 treatment was the earliest to reach the highest point in 5 th day,other treatments reached the highest in the day of 20^（th）,and T4 treatment amino acid content higher than other treatments.The membership function analysis of subordination function method for the changes in various physiological of Bupleurum chinese were as follows T4〉T3〉T2〉CK〉T1.The effects of germination were different for using four treatments of soaking germinated seeds.And 0.05 mmol·L^（-1） salicylic acid＋10 mg·mL^（-1） chitosan was better than others.
出处
《北方园艺》
CAS
北大核心
2017年第22期149-153,共5页
Northern Horticulture
基金
国家科技部子课题资助项目(2011BAI07B05-4)
河北省科技厅资助项目(16236420)
唐山市科技局资助项目(15130205C)
唐山师范学院资助项目(11PT01-2
2017B05)
关键词
北柴胡种子
水杨酸
壳聚糖
动态变化
Bupleurum chinense
salicylic acid
chitosan
dynamic change
