摘要
从感染白腐病的马铃薯中分离筛选了一株高产小核菌多糖菌株,命名为齐整小核菌SCL2010(Sclerotium rolfsii SCL2010),对菌株SCL2010发酵培养基的碳源、有机氮源进行优化,并对发酵过程中pH值的变化进行了研究。结果表明,以葡萄糖为碳源、玉米浆为有机氮源对菌株SCL2010进行发酵,小核菌多糖的产量、碳源转化率和产物合成速率分别达到28g·L^(-1)、56%和0.39g·L^(-1)·h^(-1),显著高于已有研究报道。在发酵产小核菌多糖的过程中,其pH值的变化与已有研究报道显著不同:其pH值从发酵开始迅速下降至3.0以下后开始缓慢上升至3.5~4.0;如果控制pH值则使小核菌多糖的产量显著降低,而草酸含量升高,以此推测小核菌多糖的合成可能与pH值的变化有关,后续将进行深入研究。
We isolated a scleroglucan high-yielding strain named Sclerotium rolfsii SCL2010 from potatoes suffered white rot, optimized carbon source and organic nitrogen source of fermentation medium, and investiga- ted pH value change in the fermentation process. The results showed that,in the fermentation with glucose as a carbon source and corn steep liquor as a nitrogen source, scleroglucan yield, nitrogen source conversion rate, and product synthetic rate were 28 g ~ L-1, 560/oo, and 0. 39 g ~ L 1 o h 1 respectively, which were significantly higher than those of previous studies. Different from the previous studies,the pH value dropped to below 3.0 at the initial stage while rose slowly to 3.5~4.0. When the pH value was controlled at a certain value,the sclero- glucan production decreased significantly but the oxalic acid content increased, which revealed the scleroglucan synthesis might be associated with the pH value change,and further study is required.
出处
《化学与生物工程》
CAS
2017年第11期19-22,共4页
Chemistry & Bioengineering
基金
山东省重点研发计划项目(2015GGX102015)
关键词
小核菌多糖
齐整小核菌
高产菌株
发酵特征
scleroglucan
Sclerotium rolfsii
high-yielding strain
fermentation characteristics
