摘要
目的:以储食法测量大鼠体重调定点,并探讨饮食量限制对体重调定点的影响。方法:选取雄性SD大鼠12只,随机分为对照组和实验组,每组6只。测量基线体重调定点后,对照组保持测量基线调定点饮食量,实验组给予饮食量限制(正常饮食量的70%),观察6周,之后两组均予以测量基线调定点饮食量继续观察6周。测量各时间点大鼠储食量及体重,通过线性回归法计算大鼠体重调定点。结果:大鼠体重和体重调定点随鼠龄增长而增加。限制饮食量不能降低大鼠体重调定点(P>0.05)。饮食量限制期间,大鼠体重明显下降(P<0.01),撤除饮食量限制后,大鼠体重出现反跳现象(P<0.01)。结论:饮食量限制可短期降低大鼠体重,但不能降低实验动物体重调定点,其短期体重反弹风险较高。利用储食法测量大鼠体重调定点方法稳定而可靠。
Objective： This study was aimed to use hoarding behavior to measure the body weight set-point in rats and to discuss the effect of food restriction on the body weight set-point. Methods： Twelve male SD rats were randomly divided into a control group and a treatment group （n=6 per group）. After measuring baseline set-point, the control group rats were given the same amount of food as baseline set point period and the treat- ment group rats were treated with food restriction （70% of normal diet） for 6 weeks, and the rats were monitored for additional 6 weeks after ceasing the treatment. The amount of food hoarded by the rats and their body weight were measured 6 days per week. And the hoarding behavior was measured every other week at various body weights from 0 to 12 weeks. The body weight set-point of rats was calculated by linear regression equation. Results： The body weight set-point value in both groups were continuously increased from 0 to 12 weeks, but no significant difference was found between the two groups （P〉0. 05）. During food restriction period, body weight of the treatment group rats was significantly decreased （P〈0. 01 ）, however, their body weight gained after ceasing food restriction （P〈 0. 01 ）. Conclusions： These results suggested that food restriction temporarily reduced body weight, rather than body weight set-point in rats. The behavioral method of the weight threshold to hoard food to assess body weight set point is accurate and reliable.
出处
《广西医科大学学报》
CAS
2017年第11期1664-1667,共4页
Journal of Guangxi Medical University
关键词
大鼠
体重
调定点
测量方法
储存食物
rat
body weight
set-point
measurement method
food hoarding
