摘要
目的探究氧化苦参碱(oxymatrine,OMT)对二硝基氟苯(Dinitrofluorobenzene,DNFB)诱导的ICR小鼠慢性湿疹模型的治疗作用。方法将25只ICR小鼠随机分为五组,分别为30mg·kg^(-1)氧化苦参碱组(OMT Ⅰ组)、60mg·kg^(-1)氧化苦参碱组(OMT Ⅱ组)、1mg·kg^(-1)地塞米松组、模型组及正常对照组,其中前四组利用DNFB致敏和激发建立ICR小鼠慢性湿疹模型,致敏当天分别腹腔注射30mg·kg^(-1)氧化苦参碱、60mg·kg^(-1)氧化苦参碱、1mg·kg^(-1)地塞米松及PBS溶液,正常对照组腹腔注射等量PBS溶液,每日1次,共6周。治疗前后对小鼠耳皮损进行评分,测量耳厚度变化;实验结束行耳部组织病理检查,利用HE染色对比观察各组小鼠耳皮损组织病理学变化,计算各组小鼠皮损中炎症细胞数量。结果从第2、3、4次激发后24h,OMT Ⅰ组、OMT Ⅱ组、地塞米松组小鼠皮损评分均低于模型组(P<0.05),OMT Ⅱ组与地塞米松组间差异无统计学意义(P>0.05),第4次激发后24h,评分OMT Ⅱ组低于OMT Ⅰ组(P<0.05)。从第2、3、4次激发后24h,小鼠耳厚度OMT Ⅰ组、OMT Ⅱ组、地塞米松组均小于模型组(P<0.05),OMT Ⅰ组与地塞米松组间差异无统计学意义(P>0.05),OMT Ⅱ组小于地塞米松组(P<0.05),第4次激发后24h,OMT Ⅱ组小于OMT Ⅰ组(P<0.05)。OMT Ⅰ组、OMT Ⅱ组、地塞米松组及正常对照组小鼠炎细胞数均小于模型组(P均<0.05),OMT Ⅱ组与地塞米松组间差异无统计学意义(P>0.05),OMT Ⅱ组小于OMT Ⅰ组(P<0.05)。结论氧化苦参碱可改善DNFB诱导的ICR小鼠慢性湿疹皮损,显著减轻炎症反应,疗效显著。
Objective To explore the efficacy of oxymatrine（OMT）on dinitrofluorobenzene（DNFB）-induced chronic eczema model in ICR mice. Methods Twenty-five ICR mice were randomly divided into five groups：30 mg·kg^-1 OMT（OMT Ⅰ）,60 mg·kg^-1 OMT（OMT Ⅱ）,1 mg·kg^-1 dexamethasone,model and normal control group.Chronic eczema mice model was induced by receiving DNFB in former four groups. Different doses of oxymatrine（30,60 mg·kg^-1）,dexamethasone（1 mg·kg^-1）and phosphate-buffered saline（PBS） were administrated by intraperitoneal injection on the same day after giving DNFB,respectively. One group served as the normal control was administered the same dose of PBS. Drug was administered once a day for 6 weeks. Ear skin lesions were scored and the ear thickness changes measured before and after treatment. At the end of experiment,the ear tissue of all group mice was histopathologically examined and histopathologic changes were observed. Meanwhile,the number of inflammatory cells in ear lesions were calculated. Results Beginning from 24 h after the second,third and fouth challenge,score of mice skin lesion severities in 30 mg·kg^-1 OMT,60 mg·kg^-1 OMT,1 mg·kg^-1 dexamethasone,and normal control group were significantly lower than that of model group（P〈0.05）. There was no significant difference between OMT Ⅱ group and dexamethasone group（P〈0.05）. The score of OMT Ⅱ group was significantly lower than that of OMT Ⅰ group at 24 h after the fourth challenge（P〈0.05）. Beginning from 24 h after the second,third and fouth challenge,ear thickness in 30 mg·kg^-1 OMT,60 mg·kg^-1 OMT,1 mg·kg^-1 dexamethasone,and normal control group were less significantly than that of model group（P〈0.05）. There was no significant difference between OMT Ⅰ group and dexamethasone group（P〈0.05）. Ear thickness of dexamethasone group was higher than that of OMT Ⅱ group（P〈0.05）. OMT Ⅱ group was lower significantly than OMT Ⅰ group at 24 h after the fourth challenge（P〈0.05�
出处
《宁夏医科大学学报》
2017年第9期1022-1025,1033,共5页
Journal of Ningxia Medical University
基金
宁夏医科大学校级课题(XZ201309)
