摘要
骆驼能够采食其他动物不能够食用的具有强烈气味的有毒植物,而不影响其正常生理代谢.研究发现骆驼采食的植物毒素与喹啉等杂环化合物具有相似的化学结构,所以研究骆驼体内是否存在潜在的杂环化合物降解菌具有重要意义.研究采集3头骆驼消化道内容物(瘤胃和肠道),以喹啉为唯一碳源和氮源进行5代富集培养.通过高通量测序技术对消化道内容物和5代富集培养微生物分离、筛选的菌株进行了16S r DNA PCR扩增与测序分析.从第5代富集培养液中共分离获得降解喹啉的菌17株,其中优势微生物是芽孢杆菌属的菌和苍白杆菌属,分别占到77.78%和17.60%.实验中2株降解菌K3、K38分别在132 h内降解喹啉66.79%和66.20%,显示较强的降解能力.选取菌株K3进行4因素、3水平正交实验.结果表明,温度对实验结果影响最大,其次是接种量,再次为初始pH,最后是碳源.菌株K3的最佳实验条件是温度37℃,接种量15%,初始pH 7.0,碳源5 g.
Camels can eat poisonous plants with a strong odor which other animals can＇t eat, without affecting the normal physiological metabolism.The studies show that the plant toxin that the camels gather from the food has similar chemical structures with quinoline heterocyclic compounds, so it is of great significance that whether there are potential heterocyclic compounds degrading bacteria in the camel body.Gastrointestinal contents were collect- ed from three camels＂ rumens and guts, enrichment culture was applied for five generations by using quinoline as sole carbon source and nitrogen source.High throughput sequencing （Illumina MiSeq was U~ sequence 16S rDNA PCR of microorganisms from the five dynasties enrichment and isolate cuhure.17 strains of microorgan- isms for degrading Quinoline were isolated, and the dominant species was Bacillus and Ochrobactrum, which took 77.78% and 17.60%, respectively.There were two degrading bacteria strains, K3, K38, degrading 66.79% and 66.20% of quinoline ,respectively within 132 hours,which shows strong degradation ability in the experiments.In- oeulum size shows the greatest effect in four kinds of environment factors in the experiment for K3, and the second is the temperature, then initial pH, and the carbon source.The best experimental condition is the temperature of 37 ℃ ,inoculum size of 15% ,initial pH 7.0,5 g carbon source.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第6期1073-1081,共9页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金(31160028)
四川省教育厅重点项目(17ZA0105)
成都医学院校基金(CYZ15-06)
新疆维吾尔自治区百名博士引进计划(BS2016008)
关键词
瘤胃
杂环化合物
生物降解
喹啉
rumen
heteroeyclic compounds
biodegradation
quinolone
