摘要
土壤微生物是陆地生态系统中维持土壤生产力的重要组分。因此,深入了解不同环境中土壤微生物群落功能多样性的作用,对于整个生态系统的研究具有重要意义。为了探讨油松(Pinus tabuliformis Carr.)、蒙古栎(Quercus mongolica Fisch.ex Ledeb.)混交林造林模式对土壤微生物群落功能多样性的影响,运用Biolog-Eco技术,对辽宁省建平县油松人工纯林(YC)、无植物种植土壤(NP)以及油松-蒙古栎人工混交林(YM)土壤微生物代谢功能多样性进行研究,探讨不同类型林分对土壤微生物群落的影响。结果显示:不同类型林分的土壤微生物群落代谢活性差异显著,总碳源的利用能力(AWCD)的顺序为:YM>YC>NP。不同类型林分的土壤微生物对六大碳源的利用率存在差异,YM对L-天门冬酰胺、L-苯基丙氨酸、L-丝氨酸、L-苏氨酸、吐温40、D-半乳糖酸-γ-内脂、苯乙胺、D-葡萄糖胺酸、4-羟基苯甲酸、γ-羟基丁酸的利用能力显著高于YC(P<0.05),YC又显著高于NP。主成分分析显示,糖类和氨基酸类碳源是影响微生物活性的重要碳源。3种林分类型土壤微生物Shannon指数无明显差异,物种Mc Intosh指数依次为YM>YC>NP,Simpson指数YM和YC无明显差异,二者均显著高于NP。结果表明,油松-蒙古栎混交林可以显著提高土壤微生物的活性。
Soil microorganisms play important roles in material and energy flows of terrestrial ecosystems;understanding the changes of function and structure of soil microorganisms is of great significance to the study of the whole ecosystem.In order to evaluate the effect of the mixed Pinus tabuliformis Carr.＋ Quercus mongolica Fisch.ex Ledeb.forest on soil microbial community functional diversity,by using Biolog Ecoplate system,this paper studied the functional diversity of soil microbial community under three different vegetation types in Jianping County,Liaoning Province,including pure P.tabuliformis Carr.forest（YC）,no-vegetation soil（NP）,and mixed P.tabuliformis Carr.＋ Q.mongolica Fisch.ex Ledeb.forest（YM）.The results showed that soil microbial metabolic activity（AWCD） was in the order of YM〉YC〉NP,and soil microbial metabolic activity of NP was the lowest.There were variations in carbon utilization by soil microbial communities among the three plantation forests.Soil microbes in the mixed forest had significantly higher utilization rates in L-asparagine,L-phenylalanine,L-40 serine,L-threonine,Tween-40,D-galactose acid-γ-fat,phenethylamine,D-glucose,amino acid,4-hydroxy benzoic acid than in the YC（P〈0.05）.Principal component analysis showed that carbohydrate and amino acids were important factors affecting soil metabolic activity.The Shannon＇s diversity index of soil microbes was not significantly different among the three plantation forests.The Mc Intosh＇s index was in order of YM〉YC〉NP,while there was no significant difference in Simpson＇s index between the YM and YC,and both had significantly higher Simpson＇s index than NP.Our results demonstrated that planting P.tabuliformis and Q.mongolica trees together can promote soil microbial activity.
出处
《生态学杂志》
CAS
CSCD
北大核心
2017年第11期3028-3035,共8页
Chinese Journal of Ecology
基金
辽河平原农田土壤污染防控型防护林系统构建技术研究与示范(2015BAD07B050502)
辽宁省宜林地营林决策平台构建及示范项目(201304216)
东北森林生态要素全指标观测技术研究(201404303-05)资助
