摘要
目的建立一种简便、灵敏的测定大鼠尾静脉和灌胃给予胆甾醇琥珀酰基白及多糖载紫杉醇纳米粒子后的血浆药物浓度的HPLC方法,并评价其药动学特征。方法以酮康唑为内标,采用Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-乙腈-水(45∶20∶35)为流动相,流速为1.0 ml/min,采用统计矩法计算药动学参数,评价药动学特征。结果紫杉醇在大鼠血中浓度在0.5~20μg/ml范围内线性关系良好(r=0.999 7),准确度和精密度均符合生物样品分析要求。尾静脉注射3种不同取代度的自制纳米粒和紫杉醇注射液后的t1/2分别为3.86、3.76、3.35和2.62 h;灌胃给予自制紫杉醇纳米粒溶液和紫杉醇混悬液后的t1/2分别为5.28和3.72 h。结论该测定方法可用于紫杉醇纳米粒子在大鼠体内的药动学研究,且载紫杉醇自组装纳米粒子在体内的滞留时间比紫杉醇注射液和紫杉醇混悬液有明显延长。
Objective To establish a simple and sensitive method for the determination of plasma drug concentration in rats after intragastrical and intravenous administration of paclitaxel loaded nanoparticles and to evaluate its pharmacokinetic characteristics.Methods Agilent TC-C18 column( 250 mm × 4. 6 mm,5 μm) was used for HPLC at the flow rate 1 ml/min with ketoconazole as internal standard and methanol acetonitrile water( 45∶ 20∶ 35) as the mobile phase. The statistical moment method was applied to calculate the pharmacokinetic parameters and to evaluate pharmacokinetic characteristics. Results There was a good liner relationship( r =0. 999 7) when rat blood concentration of Paclitaxel ranged from 0. 5 to 20 μg/ml. The accuracy and precision were in line with the requirements of biological sample analysis. The t1/2 were 3. 86,3. 76,3. 35 and 2. 62 h after intravenous injection of three lab-made paclitaxel nanoparticles and paclitaxel solution via rat tail vein. The t1/2 were 5. 28 and 3. 72 h respectively after intragastrical administration of lab-made paclitaxel nanoparticles and paclitaxel suspension. Conclusion This method can be used for the pharmacokinetic study of paclitaxel nanoparticles in rats. The paclitaxel loaded nanoparticles exhibited significantly longer in vivo retention time than paclitaxel injection and paclitaxel suspension.
作者
张琦
赵文萃
刘洋
张公良
成念
侯吉华
ZHANG Qi;ZHAO Wencui;LIU Yang;ZHANG Gongliang;CHENG Nian;HOU Jihua(Jiangxi University of TCM , Nanchang 330004 , China;No .208 Hospital of PLA ,Changchun 130062 ,China;Changchun Cadres＇s Sanatorium ,Chang-chun 130052 ,China;Yanbian University ,Yanji 133000 ,China)
出处
《药学实践杂志》
CAS
2017年第6期504-507,共4页
Journal of Pharmaceutical Practice
基金
国家自然科学基金(81173559)
吉林省卫生计生委基金(2015ZC040)
关键词
白及多糖
紫杉醇
纳米粒子
药动学
Rhizoma Bletillae polysaccharide
paclitaxel
nanoparticles
pharmacokinetics
