摘要
目的初探淡豆豉对人体肠道6种常住菌的调节作用。方法利用选择性培养基从人的粪便中分离培养肠杆菌、肠球菌、双歧杆菌、乳杆菌、产气荚膜梭菌和脆弱拟杆菌6种人体肠道常住菌。将高、中和低浓度淡豆豉药液加入选择性培养基中,以不加药液的培养基作为空白对照,根据各菌种培养条件分别于厌氧或好氧条件下培养,然后进行菌落计数。结果与空白对照相比,加药培养基脆弱拟杆菌增长20.00%以上;而其他5种菌的生长均被不同程度的抑制,其中对乳杆菌和肠球菌抑制作用最强,加药培养基上这2种菌均不生长;50.00%浓度药液对产气荚膜梭菌抑制率为32.81%;20.00%浓度药液对肠杆菌完全抑制,但随着药液浓度增大抑制作用减弱,50.00%和70.00%浓度药液对肠杆菌的抑制率分别为53.83%和22.11%;低、中和高浓度药液对双歧杆菌的抑制率分别为17.07%、17.83%和17.84%,差异无统计学意义。结论淡豆豉对人体6种肠道常住菌具有不同程度的调节作用。
Objective To investigate the regulatory effect of Sojae Semen Praeparatum（SSP）on 6 common gut microbes.Methods Selective culture medium was used to isolate and culture 6 gut microbes（Enterobacter,Enterococcus,Bifidobacterium,Lactobacillus,Clostridium perfringens and Bacteroides）from human faeces.High,medium and low concentrations of liquid SSP extract were added to the selective media respectively,with the media without SSP liquid as the controls.Then the microbes were cultured with the media under anaerobic or aerobic conditions according to their characteristics,and the number of colony on each medium was counted.Results Compared with the blank control,Bacteroides increased by more than20.00% on the media containing SSP liquid,while the other 5 bacteria were inhibited at different degrees,among which Lactobacillus and Enterococcus were inhibited most highly with no visible bacterial colonies.The inhibition ratio of 50.00% SSP liquid against Clostridium perfringens was 32.81%.20.00% SSP liquid completely inhibited Enterobacter,but the inhibition weakened with the concentration increasing.The inhibition ratios of 50.00% and 70.00% SSP liquid against Enterobacter were 53.83% and 22.11%respectively.The inhibition ratios of low,medium and high concentration of SSP against Bifidobacterium were17.07%,17.83% and 17.84% respectively,with no significant differences.Conclusion SSP has different degrees of regulatory effect on 6 common gut microbes.
出处
《中国微生态学杂志》
CAS
CSCD
2017年第10期1122-1126,共5页
Chinese Journal of Microecology
基金
国家中医药管理局公益性行业科研专项经费项目(201507004-03)
黑龙江省应用技术研究与开发计划新创办企业项目(PB15F005)
关键词
淡豆豉
肠道菌群
常住菌
调节作用
Sojae semen praeparatum
Gut microbiota
Resident flora
Regulatory effect
