摘要
目的:miR-107靶向NID2调控Notch信号通路影响肺癌的侵袭和增殖。方法:免疫组化检测NID2在肺癌组织和正常肺组织中的表达;PCR检测miR-107在肺癌组织中的表达;双荧光素酶报告基因系统检测miR-107对NID2转录活性的影响;肿瘤细胞成球实验检测miR-107的表达对肺癌细胞A549的增殖能力的影响;Transwell侵袭实验检测miR-107的表达对肺癌A549细胞的侵袭能力的影响;划痕试验检测miR-107的表达对肺癌A549细胞的迁移能力的影响;Western blot检测过表达miR-107后Notch信号通路的蛋白表达水平。结果:和正常肺组织比较,NID2在肺癌组织中表达较高;miR-107在肺癌组织中表达明显降低;双荧光素酶报告基因系统检测结果显示,miR-107可以直接调控NID2的转录活性;过表达miR-107后,肺癌细胞A549的增殖、侵袭和迁移能力明显降低;过表达miR-107后,Notch1、hes-1、presenilin1蛋白表达下调。结论:miR-107靶向NID2的表达,通过Notch通路调控肺癌细胞的增殖和侵袭能力。
Objective：To investigate the effect of miR-107 targeting NID2 on Notch signaling pathway on invasion and proliferation of lung cancer.Methods：The expression of NID2 in lung cancer and normal tissues was detected by immunohistochemistry.The expression of miR-107 in lung cancer cell line was detected by PCR.The effect of miR-107 on the expression of NID2 was examined by the dual luciferase gene system.Effect of miR-26 on the proliferation ability of H1650 were detected by tumor in ball assays.Transwell invasion assay was used to detect the ability of invasion in the lung cancer cell line A549 after overexpression the miR-107.Scratch test was used to detect the ability of migration in the lung cancer cell line A549 after overexpression the miR-107.Western blot was used to detect the protein expression of Notch signal pathway after overexpression miR-107.Results：The expression of NID2 in lung cancer tissues was significantly higher than that in normal lung tissues.The expression of miR-107 in lung cancer tissues was significantly lower than that in normal lung tissues.The dual luciferase reporter gene system showed that miR-107 could directly regulate the transcriptional activity of NID2.After miR-107 overexpression,the proliferation,invasion and migration ability of lung cancer cell A549 were significantly decreased.The expression of miR-107 protein could down-regulate the expression of Notch1,hes-1 and presenilin1 protein.Conclusion：miR-107 targets the expression of NID2 and regulates the invasion and proliferation of lung cancer cells by Notch pathway.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第11期1662-1667,共6页
Chinese Journal of Immunology
