摘要
目的探讨妊娠期高血压疾病孕妇与正常孕妇胎盘和外周血Th17与Treg细胞及相关细胞因子的差异,探讨Th17与Treg细胞及相关细胞因子与妊娠期高血压疾病的相关性。方法选取该院2015年1月-2016年12月收纳的60例妊娠期高血压疾病孕妇作为观察组,选取同期在该院妇科及门诊进行孕检的60例正常孕妇作为对照组,用流式细胞仪检测两组孕妇外周血中Th17与Treg细胞含量,用实时定量PCR法检测外周血中相关细胞因子IL-2、IL-4、IL-10、IL-21的含量与胎盘组织中miRNA-21表达量。结果观察组孕妇外周血中Th17细胞在T细胞中的占比高于对照组(P<0.05)。观察组孕妇外周血中Treg细胞在T细胞中的占比低于对照组(P<0.05)。观察组孕妇外周血中Th17细胞与Treg细胞的比值高于对照组(P<0.05)。观察组孕妇外周血中IL-2、IL-21细胞因子含量高于对照组(P<0.05)。观察组孕妇外周血中IL-4、IL-10细胞因子含量低于对照组(P<0.05)。观察组孕妇胎盘组织中miRNA-21相对表达量高于对照组(P<0.05)。结论定期监测Th17、Treg细胞及相关细胞因子IL-2、IL-4、IL-10、IL-21等指标,有助于预测妊娠期高血压疾病孕妇的病情发展,对早期妊娠期高血压疾病患者积极采取治疗措施改善病情,若符合剖宫产指征,应及时终止妊娠。在孕妇常规孕检时检测Th17、Treg细胞与相关细胞因子等指标,可早期发现、预防妊娠期高血压疾病的病情发展,把握治疗时期,改善患者预后及母婴结局。
Objective To explore the differences of Th17,Treg cells and related cytokines in placenta and peripheral blood of patients with hypertensive disorder complicating pregnancy（ HDCP） and normal pregnant women,analyze the correlations between Th17,Treg cells,related cytokines and HDCP. Methods Sixty pregnant women with HDCP were selected from Gansu Provincial Maternal and Child Health Care Hospital from January 2015 to December 2016 as observation group,and sixty normal pregnant women receiving prenatal examination in Department of Gynecology and Outpatent Department in the hospital during the same period were selected as control group. Flow cytometry was used to detect the levels of Th17 and Treg cells in peripheral blood of pregnant women in the two groups. Real-time quantitative PCR was used to detect the levels of related cytokines（ IL-2,IL-4,IL-10,IL-21） in peripheral blood and expression level of miRNA-21 in placenta. Results The proportion of Th17 cells of T cells in peripheral blood of pregnant women in observation group was statistically significantly higher than that in control group（ P0. 05）. The proportion of Treg of T cells in peripheral blood of pregnant women in observation group was statistically significantly lower than that in control group（ P0. 05）. The ratio of Th17/Treg cells in peripheral blood of pregnant women in observation group was statistically significantly higher than that in control group（ P0. 05）. The levels of IL-2 and IL-21 in peripheral blood of pregnant women in observation group were statistically significantly higher than those in control group（ P0. 05）. The levels of IL-4 and IL-10 in peripheral blood of pregnant women in observation group were statistically significantly lower than those in control group（ P0. 05）. The relative expression level of miRNA-21 in placenta of pregnant women in observation group was statistically significantly higher than that in control group（ P0. 05）. Conclusion Regular monitoring of Th17,Treg cells and related cytoki
出处
《中国妇幼保健》
CAS
2017年第21期5215-5218,共4页
Maternal and Child Health Care of China
基金
甘肃省卫生科研计划项目(GSWSKY-2014-20)
关键词
妊娠期高血压疾病
孕妇
胎盘
外周血
Th17细胞
TREG细胞
细胞因子
Hypertensive disorder complicating pregnancy
Pregnant women
Placenta
Pelipheral bloodL Thl7 cell
Treg cell
Cyto-kine
