摘要
随着魁蚶养殖规模的发展,产生了大量以脏器为主的副产物,为综合利用这些加工副产物,以魁蚶为原料,通过单因素和正交试验确定乙醇回流法提取牛磺酸的工艺条件,并利用高效液相色谱法测定了含量。最佳提取工艺为料液比1 g∶12 m L,乙醇体积分数75%,提取时间50 min,温度70℃,牛磺酸的提取量为5.36mg/g,牛磺酸在5.0~20.0μg/m L浓度范围内有良好线性关系,相关系数R2为0.999 7,回收率为89.27%~97.27%,相对标准偏差1.83%,表明高效液相色谱法测定水产品中牛磺酸含量准确性高、重现性好。魁蚶内脏中牛磺酸提取量最高,为6.29 mg/g,斧足次之,血水最少,表明魁蚶中牛磺酸含量丰富,在不同组织中存在差异,为魁蚶的开发利用开辟了新途径。
With the development of Scapharca broughtonii breeding scale,a large number of viscera byproducts were produced. In order to comprehensively utilize these byproducts,this study took Scapharca broughtonii as raw material,confirmed the taurine extraction process condition by ethanol refluxing through single factor and orthogonal test,and measured its content by high performance liquid chromatography（ HPLC）. The optimal extraction conditions were：solid-liquid ratio 1 g ∶12 m L,ethanol concentration 75%,extraction time 50 min,temperature 70℃,the extraction amount 5.36 mg/g. The taurine was in the range of 5. 0 ~ 20. 0 μg/m L with good linear correlation,the correlation coefficient R2= 0.999 7,the recoveries were 89.27% ~ 97.27% and the RSD was 1.83%,which indicated that HPLC had determined the taurine content in aquatic products with high accuracy and good reproducibility. The taurine extraction content was 6.29 mg/g,which was the highest in viscera of Scapharca broughtonii,foot took second place,blood was the lowest. These results suggested that the taurine content in Scapharca broughtonii was rich and varied in different tissues. This study opened up a new way for the development and utilization of Scapharca broughtonii.
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2017年第11期132-138,共7页
Journal of Agricultural Science and Technology
基金
国家海洋局公益性行业专项(201505030)资助
关键词
魁蚶
牛磺酸
提取
Scapharca broughtonii
taurine
extraction
