摘要
【目的】克隆甘蔗ASR(ABA-stress-ripening)基因的序列并检测其在干旱胁迫下的表达量,为甘蔗抗逆胁迫机制的研究提供实验依据。【方法】以甘蔗叶片总RNA为模板,通过RT-PCR扩增ASR基因的cDNA,采用生物信息学软件分析所克隆基因的编码蛋白特性,并用荧光定量PCR分析该基因的表达情况。【结果】克隆得到的cDNA片段长度为402 bp,编码133个氨基酸,命名为SoASR1(GenBank登录号:JQ712581)。同源性分析表明,SoASR1基因在12种植物中的一致性为56%~99%。实时荧光定量分析结果表明,随着甘蔗干旱胁迫时间的延长,SoASR1基因表达量先升高后下降,干旱胁迫加硅处理的甘蔗叶片SoASR1基因表达量峰值比干旱处理提前34 h出现。【结论】克隆获得甘蔗SoASR1基因,SoASR1基因受到干旱胁迫的诱导表达,在转录水平上参与了甘蔗抗干旱胁迫反应,可作为候选基因用于甘蔗抗旱机制研究。施硅能进一步提高甘蔗抗旱性。
【Objective】To provide experimental basis for further research on regulation mechanism of ASR in response to drought,ASR（ ABAstress-ripening） gene from sugarcane was cloned and its expression level under drought stress was detected.【Method】Based on the total RNA of sugarcane leaves,the cDNA of ASR gene was amplified by RT-PCR,the characteristics of the deduced protein were analyzed by using bioinformatics software and its expression was analyzed by quantitative real-time PCR. 【Result】The results showed that the cDNA of ASR gene which obtained by RT-PCR,was 402 bp in full length,and it encoded a putative ASR protein with 133 amino acids,named SoASR1,its GenBank accession number was JQ712581. Comparison of the amino acids sequences homology in SoASR1 protein from 12 different species indicated that the ASR protein had 56 % to 99 % identity in amino acids sequence with other plants. The results of quantitative realtime PCR analysis showed that the mRNA of SoASR1 was increased initially and then decreased with extension of time under drought stress.The peak value of SoASR1 gene expression in sugarcane leaves treated with PEG and Si was 34 h earlier than that of drought treatment.【Conclusion】SoASR1 was induced by drought stress and was involved in drought-resistant reaction at transcriptional level,so it could be served as a candidate gene for further study of drought-resistant mechanism of sugarcane,which could be enhanced by Si application.
作者
梁潘霞
邢颖
廖青
黄杏
李长宁
黄太庆
江泽普
李杨瑞
LIANG Pan-xia;XING Ying;LIAO Qing;HUANG Xing;LI Chang-ning;HUANG Tai-qing;JIANG Ze-pu;LI Yang-mi(Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi Nanning 530007 , China;Sugar-cane Research Center, Chinese Academy of Agricultural Sciences/ Key Laboratory of Sugarcane Biotechnology and Genetic Improvement （Guangxi） , Ministry of Agriculture/ Guangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Nanning 530007, China)
出处
《西南农业学报》
CSCD
北大核心
2017年第10期2196-2201,共6页
Southwest China Journal of Agricultural Sciences
基金
广西自然科学基金项目(2014GXNSFBA118139
2016GXNSFBA380131)
广西农业科学院科技发展基金项目(桂农科2017JM03
桂农科2017JM01
桂农科2015JM23)
南宁市西乡塘区科学研究与技术开发计划项目(201710304)
关键词
甘蔗
干旱胁迫
ASR基因
Sugarcane
Drought
ABA-stress-ripening
