摘要
为了解河南省奶牛传染性鼻气管炎病毒(Infectious bovine rhinotracheitis virus,IBRv)的感染情况,采用SYBR Green I荧光定量PCR方法,对郑州、新乡、焦作等地区采集的397头奶牛鼻腔、阴道棉拭子进行了IBRv DNA检测。结果:总体检出率为7.1%,检出率最高场可达73.7%;患病牛群中的检测率(6.5%)高于外观健康牛群中的阳性率(5.9%)。由此证实,河南省奶牛场中存在一定的IBRV感染,并且其感染程度与年龄、机体健康状况、牛场饲养管理和卫生情况具有一定的相关性。
To understand the prevalence of IBR in Henan Province. Using quantitative PCR with SYBR Green I nasal cavity and vaginal swab of f97 cows collected from Zhengzhou,Xinxiang,Jiaozuo and other regions were detected. Re-sults ：the total positive rate was 7. 1% ,the highest farm reached 73. 7% ; the diseased herd （6. 5% ） ,compared the 5.9% in the healthy appearance herd. Conclusion： There is a certain infection of IBRV in dairyfarms in Henan province,and the prevalence has certain correlation with age,heahealth of cattle.
出处
《现代牧业》
2017年第1期20-22,共3页
Modern Animal Husbandry
关键词
奶牛
传染性鼻气管炎
Cow
infectious bovine rhinotracheitis
quantitative PCR
