摘要
采用丝素蛋白(SF)为原料,将枯草菌脂肽钠(SS)与丝素蛋白混合以缩短丝素蛋白溶液凝胶时间,并在凝胶过程中对SF/SS共混溶液施加机械剪切力,制备一种取向的SF/SS水凝胶。结果表明:在剪切速率为55 s^(-1)下,当剪切时间为0~20 min时,SF/SS混合体系中分子构象呈现大量的无规卷曲,而在20 min至凝胶完全时,分子构象由无规卷曲向β-折叠结构转变;剪切诱导形成的SF/SS水凝胶具有显著的取向网状形貌,其取向方向的压缩强度是非取向方向、未剪切水凝胶的3.5倍,其垂直于取向方向的耐切割性能是未剪切、取向方向的2倍。SF/SS取向水凝胶可用于神经细胞、骨细胞的培养,以及缺陷肌肉、韧带组织的修复。
In this study,silk fibroin( SF) was adopted as raw material,bacillus subtilis sodium surfactin( SS) was blended with silk fibroin to shorten the gelation time of SF solution,and mechanical shear force was exerted on the blended solution of SF/SS in the gelation process to prepare an orientational SF/SS hydrogel. The results suggest that the molecular conformation of the SF/SS blending system takes on random coil it is subject to shearing for 0 min to20 min at the rate of 55 s^(-1),while the molecular conformation transforms from random coil into β-sheet conformers during the period that it is subject to shearing for 20 min until completely gelling; the shear-induced SF/SS hydrogels appears to be of obvious orientational gel skeleton/network morphology,its compressive strength in the direction of orientation is 3. 5 times over that of unsheared hydrogel in the direction of non-orientation,and its cut resistance in the direction vertical to the direction of orientation is twice over that of unsheared hydrogel in the direction of orientation. SF/SS orientational hydrogels can be applied for culture of nerve cell and bone cell,and repairing of defect in muscle and ligament tissues.
出处
《丝绸》
CAS
CSCD
北大核心
2017年第8期1-7,共7页
Journal of Silk
基金
国家自然科学基金项目(51373114)
江苏省高校自然科学研究重大资助项目(15KJA540001)
关键词
丝素蛋白
枯草菌脂肽钠
剪切力
取向性
水凝胶
机械性能
silk fibroin
bacillus subtilis sodium surfactin
shear force
orientation
hydrogels
mechanical property
