摘要
目的探讨针刺印堂百会风池肾俞穴组治疗抑郁症的可能作用机制。方法 40只SD大鼠随机分为正常组、模型组、针刺组、西药组,每组10只。正常组大鼠正常饲养,其余各组大鼠孤养并进行28天的不可预见的慢性应激刺激建立慢性应激性大鼠模型。造模同时模型组每日予2 ml生理盐水灌胃;针刺组选用印堂、百会、风池(双侧)、肾俞(双侧)进行针刺治疗,每次30 min,每日1次;西药组给予氟西汀胶囊0.18 mg/(kg·d)灌胃,每日1次,各组均灌胃28天;正常组正常饲养不灌胃。检测各组大鼠海马脑源性神经营养因子(BDNF)、环磷腺苷效应元件结合蛋白(CREB)mRNA含量及海马BDNF I-17位点、BDNF I-17 Cp G6位点基因甲基化水平。结果与正常组比较,模型组大鼠海马CREB、BDNF mRNA含量水平明显降低(P<0.05或P<0.01);针刺组和西药组较模型组大鼠海马CREB、BDNF mRNA含量增加(P<0.05)。各组大鼠BDNF I-17位点甲基化水平未出现明显差异(P>0.05),在BDNF I-17 Cp G6位点上模型组大鼠甲基化水平明显低于正常组(P<0.05),其他各组未出现明显差异。结论针刺印堂百会风池肾俞穴组可有效增加慢性应激模型大鼠海马内CREB、BDNF mRNA水平,可能是其抗抑郁机制之一。
Objective To explore the possible mechanism of needling points group of Yintang（ EX-HN3）-Baihui（ DU20）-Fengchi（ GB20）-Shenshu（ BL23） in treating depression. Methods Forty Sprague Dawley（ SD） rats were randomized into normal group,model group,needling group and the western medicine group,with 10 rats in each group. Rats in the normal group were fed normally. Rats in other groups were fed alone and were made chronic stress rat models by 28 days of unpredictable chronic stress stimuli. At the same time of modeling,the model group was given normal saline 2 ml by gavage every day. The needling group was given 30 minutes of acupuncture treatment in Yintang（ EX-HN3）,Baihui（ DU20）,bilateral Fengchi（ GB20） and bilateral Shenshu（ BL23） once every day. The western medicine group was given fluoxetine capsule 0. 18 mg/（ kg ·d） by gavage once every day.Rats in other groups were treated by gavage for 28 days except the normal group. It was detected that rats＇ hippocampus brain-derived neurotrophic factor（ BDNF）, adenosine cyclophosphate responsive element-binding protein（ CREB） mRNA level,as well as the methylation levels of hippocampus BDNF I-17 locus gene and BDNF I-17 Cp G6 locus gene. Results Compared with the normal group,rats＇ hippocampus CREB and BDNF mRNA level in the model group decreased（ P〈 0. 05 or P 〈0. 01）. Compared with the model group,rats＇ hippocampus CREB and BDNF mRNA level in the needling group and the Western medicine group increased（ P〈 0. 05）. The difference of methylation levels of rats＇ BDNF I-17 locus in all groups was not significant（ P 〉0. 05）. The methylation level of rats＇ BDNF I-17 Cp G6 locus in the model group was lower than that in the normal group（ P〈 0. 05）; the difference among other groups was not significant. Conclusion Needling points group of Yintang-Baihui-Fengchi-Shenshu might increase the levels of chronic stress model rats＇ hippocampus CREB and BDNF mRNA,which might be one of the mechanisms of trea
出处
《中医杂志》
CSCD
北大核心
2017年第21期1860-1863,共4页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81202766)
关键词
抑郁症
针刺
印堂百会风池肾俞穴组
脑源性神经营养因子
环磷腺苷效应元件结合蛋白
基因甲基化
depression
needling
points group of Yintang （EX-HN3） -Baihui （DU20） -Fengchi （GB20） - Shenshu （BL23）
brain-derived neurotrophic factor
adenosine cyclophosphate responsive element-binding protein
gene methylation
