摘要
本研究从贵州福泉瓮福磷矿矿区广泛采样,分离得到一株解钾菌株,命名为FP.18。通过细菌形态学观察,16S rDNA同源性分析,NCBI细菌库对比,发现该细菌与肠杆菌属(Enterobacteriaceae bacteriium)同源性达到99%,确定该菌株为肠杆菌属。煤矸石营养成份丰富,但目前未得到有效利用。利用FP.18解钾菌在温度为30℃,p H为7,接菌量为30 m L,处理天数为2 d,煤矸石目数为过120目的条件下,该菌能有效解离煤矸石,提高煤矸石中速效钾的含量。
A strain of potassium bacteria was isolated from the Wengfu phosphate mine in Fuquan,Guizhou Province,and named as FP.18. According to the results of the bacterial morphological observation,phylogenetic analysis of 16 S r DNA genes,comparison to NCBI bacterial library,it is found that the bacteria and Enterobacteriaceae（ Enterobacteriaceaebacteriium） homology reached 99%,it is determined that the strain is enterobacter. Coal gangue is rich in nutrients,but has not been effectively used. From the temperature of 30 ℃,p H 7,inoculation amount of 30 m L,the number of treatment days for 2d,and the coal gangue mesh of over 120,the bacteria could effectively dissociate coal gangue and improve the content of available potassium in gangue.
作者
李春钢
钟艳
李夏夏
姜雄
谢承卫
LI Chungang ZHONG Yan LI Xiaxia JIANG Xiong XIE Chengwei(College of Chemistry and Chemical Enigeering, Guizhou University, Guiyang 550025, Chin)
出处
《贵州大学学报（自然科学版）》
2017年第4期132-135,共4页
Journal of Guizhou University:Natural Sciences
基金
贵州省科技厅2016年科技支撑计划(黔科合[2016]支撑2808号)
贵州大学引进人才科研项目(贵大人基合字[2013]55号)
关键词
解钾菌
筛选鉴定
肠杆菌属
煤矸石
Potassium bacteria
isolation and identification
enterobacteriaceae bacterium
coal gangue
