摘要
Red/ET同源重组技术(Red/ET recombineering)是由来源于大肠杆菌λ噬菌体的蛋白对Redα/Redβ或来源于Rac原噬菌体的蛋白对Rec E/Rec T所介导的基于短同源臂(40–50 bp)的同源重组技术,能对宿主DNA序列进行快速、高效、精确的修饰和操作。本文主要综述了2010年以来Red/ET同源重组技术在大肠杆菌及其他细菌中的研究进展,同时简要介绍了该技术在微生物基因组挖掘,尤其是在微生物基因簇的异源表达领域的应用进展。
Red/ET recombineering is an in vivo homologous recombination-based genetic engineering method used primarily in Escherichia coli by using short homology arms（40–50 bp）, based on the expression of either redα/redβ from the Red operon of λ phage or the analogous rec E/rec T from Rac prophage that are located in the E. coli chromosome. It can rapidly, efficiently and accurately modify and manipulate genomic and episome DNA. In this review, we introduce the progress of Red/ET recombineering in E. coli and other bacteria, and its application in microbial genome mining, especially in the field of heterologous expression of microbial biosynthetic gene clusters.
作者
郑文韬
张友明
卞小莹
Wentao Zheng Youming Zhang Xiaoying Bian(Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Qingdao 266237, Shandong Province, Chin)
出处
《微生物学报》
CAS
CSCD
北大核心
2017年第11期1735-1746,共12页
Acta Microbiologica Sinica
基金
国家自然科学基金(31500033
31670097
31670098)
国家重点研发计划(2017YFD0201405)~~
