摘要
目的研究肥胖相关基因(FTO)与叉头转录因子O1(FoxO1)蛋白在非酒精性脂肪肝模型大鼠肝脏中的表达水平。方法通过饲喂高能量和高脂肪的大鼠饲料制备非酒精性脂肪肝动物模型,然后采集大鼠的血液和肝脏组织,测定其肝脏指数和血液生化指标,包括三酰甘油(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(AST)、天冬氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL);对大鼠进行肝脏病理检测;采用免疫组织化学法测定FTO蛋白和FoxO1蛋白在大鼠肝脏中的表达水平。结果 8周后,模型组大鼠的肝脏质量、体质量和肝脏指数3项指标均高于对照组大鼠,差异有统计学意义(P<0.05);模型组大鼠的AST、ALT、LDL、ALP、TG和TC均高于对照组大鼠,差异有统计学意义(P<0.05);模型组大鼠的HDL低于对照组大鼠,差异有统计学意义(P<0.05);模型组大鼠肝小叶部分产生脂肪变性、炎症,对照组大鼠则没有;模型组大鼠的FTO蛋白和FoxO1蛋白在肝脏中的表达积分高于对照组大鼠,差异有统计学意义(P<0.05)。结论 FTO与FoxO1一起相互作用,可能扰乱正常的能量和脂肪代谢。
Objective To research the obesity-related gene（FTO）and forkhead transcription factors O1（FoxO1）protein expression level in the livers of non-alcoholic fatty liver disease（NAFLD）rat model.Methods The animal model of NAFLD in rats was prepared by feeding high energy and high fat feed.Then the rat blood and liver tissue were collected for detecting the liver index and blood biochemical indexes,including triglycerides（TG）,total cholesterol（TC）,alanine aminotransferase（AST）,aspartate aminotransferase（ALT）,alkaline phosphatase（ALP）,high-density lipoprotein（HDL）and low density lipoprotein（LDL）;the liver pathological examination was performed;FTO protein and Fox O1 protein expression levels in rat liver were detected by using the immunohistochemical assay.Results The rat liver weight,body weight and liver index after 8 weeks in the model group were higher than those in the control group,the difference was statistically significant（P〈0.05）;the levels of AST,ALT,LDL,ALP,TG and TC in the model group were higher than those in the control group,the difference was statistically significant（P〈0.05）,the HDL level in the model group was lower than that in the control group,the difference was statistically significant（P〈0.05）;the model group produced steatosis and inflammation in hepatic lobule part,while the control group had no these lesions;the FTO protein and FoxO1 protein expression levels in liver of the model group were higher than those in the control group,the difference was statistically significant（P〈0.05）.Conclusion FTO and FoxO1 interaction may disturb the normal energy and fat metabolism.
出处
《重庆医学》
CAS
北大核心
2017年第30期4185-4186,4189,共3页
Chongqing medicine
基金
海南省卫生与计划生育委员会基金资助项目(14A210192)
海南省自然科学基金资助项目(814310)
海南省中药现代化专项(2015ZY02)
关键词
叉头转录因子类
非酒精性脂肪肝
能量代谢
脂类代谢
肥胖相关基因
blood
liver tissue
blood biochemical indexes
triglycerides（TG）
total cholesterol（TC）
immunohistochemical assay
