摘要
目的探讨常规水浴与低温水浴两种不同温度方式对诱导痰细胞分类计数以及对部分痰上清炎症介质的影响。方法收集呼吸专科门诊患者并进行诱导痰检测,将每例痰标本均分至DDT常温水浴(37℃,15 min)处理组及DTT低温水浴(0℃,60 min)处理组,统一进行细胞分类计数,采用酶联免疫吸附(ELISA)法测定白细胞介素IL-4、IL-5、IL-8的浓度。结果共收集20例成功诱导痰标本,其中男7例,女13例。低温水浴处理组痰细胞总数低于常温水浴处理组(P<0.05),但两组间痰细胞分类计数未见明显差异(P>0.05),两组痰上清液IL-4、IL-5及IL-8的浓度亦未见明显差异(均P>0.05)。结论除了低温水浴处理组获取的细胞总数相对较少以外,两种处理方法在细胞分类计数以及部分炎症介质检测上差异不大。
Objective To assess the effects of two different methods on differential cell count and thedetection on inflammatory mediators in the process of induced sputum. Methods Induced sputum samples fromoutpatients with respiratory diseases were collected. Each sample was equally divided into two parts. One portionwas processed at a regular way by incubation at 37 ℃ for 15 min in 0.1% dithiothreitol（DTT）. Another wasprocessed at 0 ℃ for 60 min in 0.1% DTT. The cell pellet was used for the cytology analysis by HE staining. Thelevels of IL-4,IL-5 and IL-8 were measured by Elisa Kit. Results Induced sputum was successfully collectedfrom 20 subjects with chronic airway diseases,including 7 males and 13 females. Total cell counts was lower in0 ℃-incubated group than in 37 ℃-incubated group（P 0.05）. However,there was no difference in differentialcell counts between two groups,as well as the levels of IL-4,IL-5 and IL-8 in sputum supernatants（all P 0.05）. Conclusions Except a difference in the total cell number,there was no difference between two methodson the differential cell count and levels of certain inflammatory mediators.
出处
《实用医学杂志》
CAS
北大核心
2017年第20期3410-3413,共4页
The Journal of Practical Medicine
基金
广东省科技计划项目(编号:2013B021800304)
广东省自然科学基金项目(编号:2015A030311045)
关键词
诱导痰
炎症介质
细胞分类
裂解方式
induced sputum
cytology
inflammatory mediators
processing method
