摘要
目的:探讨芦丁在氧化低密度脂蛋白(ox-LDL)诱导的血管平滑肌细胞(VSMC)增殖中的作用。方法:制备ox-LDL,采用贴壁法分离培养C57BL/6J小鼠的VSMC,将细胞分为对照组、芦丁组、ox-LDL组和芦丁+ox-LDL组,MTT法检测各组VSMC细胞增殖活性,Western blot检测VSMC中磷酸化细胞外信号调节激酶(p-ERK)的表达情况。结果:芦丁组与对照组细胞增殖活性及p-ERK蛋白表达水平均无明显差异(P均>0.05);ox-LDL组细胞增殖活性及p-ERK蛋白表达水平均显著高于对照组(P均<0.05);与ox-LDL组相比,芦丁+ox-LDL组细胞增殖活性及p-ERK蛋白表达水平均明显降低(P均<0.05)。结论:芦丁可能通过抑制ERK信号通路,抑制ox-LDL诱导的VSMC的增殖。
Objective：To investigate the role of rutin in the proliferation of vascular smooth muscle cells（VSMCs）induced by oxidized low-density lipoprotein（ox-LDL）. Methods：Ox-LDL was prepared and the VSMCs of C57 BL/6 J mice were isolated and cultured by adherence method.The cells were divided into control group,rutin group,ox-LDL group and rutin＋ox-LDL group.The proliferative activity of VSMCs was detected by MTT assay.Western blot was used to detect the expression of phosphorylated extracellular signal-regulated kinase（p-ERK）in VSMCs. Results：The cell proliferation activity and p-ERK expression between rutin group and control group were not significantly different（both P〉 0.05）.The cell proliferation activity and p-ERK expression of ox-LDL group were significantly higher than those of control group（both P〈0.05）.Compared with ox-LDL group,the cell proliferation activity and p-ERK expression of rutin＋ox-LDL group were significantly decreased（both P〈0.05）. Conclusion：By inhibiting ERK signaling pathway,rutin can inhibit the proliferation of VSMC induced by ox-LDL.
出处
《国际心血管病杂志》
CAS
2017年第5期299-301,共3页
International Journal of Cardiovascular Disease
基金
广东省自然基金(2014A030310207)
