摘要
【目的】探究复方天然植物制剂对奶牛回乳效果及回乳期奶牛血清胰岛素(INS)、氢化可的松(HC)、生长激素(GH)和胰岛素生长因子1(IGF-1)含量的影响。【方法】试验于2016年1—6月进行,选择四川省某规模化奶牛场半封闭统一舍饲,体重(582±41)kg、第2—4胎中国荷斯坦奶牛150头。从中选取体况良好,健康,乳房、乳汁均正常,即将进入回乳期,产奶量为(15.42±0.71)kg的妊娠后期即将干乳的健康奶牛80头。随机分为A、B、C、D组,每组20头,均采用逐渐干奶法回乳,回乳开始当天记为第0天,A组为对照组,不饲喂复方天然植物制剂。B、C、D组均从回乳开始第1天,每日上午8:00分别饲喂复方天然植物制剂400、500、600g,回乳完成后停止饲喂复方天然植物制剂。复方天然植物制剂由麦芽,朴硝,升麻,柴胡,香附,薏仁,蚕蜕,白术,黄芩,知母,苏梗,芡实,五味子,蒲公英,甘草等按一定比例组成。分别采集奶牛回乳期第0、1、3、5、7、9和11天尾静脉血,置于未加抗凝剂的离心管中,室温下静置1h,置离心机352×g离心10min,转移上层血清于EP管中,-20℃冻存。采用双抗体夹心酶联免疫吸附技术(ELISA)法检测血清INS、HC、GH和IGF-1含量,并记录回乳期奶牛单日产奶量。【结果】A、B、C、D 4组奶牛回乳时间分别为11、7、5、5天。各组回乳期奶牛血清IGF-1和HC含量均呈下降趋势,且对照组下降趋势较各复方天然植物制剂组慢,复方天然植物制剂组血清IGF-1、HC和GH含量在回乳期后回升,对照组血清INS含量上升趋势较各复方天然植物制剂组慢,第0至1天,各组奶牛血清HC、IGF-1和GH含量差异均不显著(P>0.05);第3—7天,A组奶牛血清HC、GH和IGF-1含量极显著高于B、C、D组(P<0.01),而血清INS含量极显著低于B、C、D组(P<0.01);第9天,A、D组奶牛血清HC含量均显著高于C组(P<0.05),A、B组奶牛血清GH和IGF-1含量均极显著低于C、D组(P<0.01),A组奶牛血清
【Objective】the objective of this study is to explore the impact of compound natural plant preparation on the milk withdrawal and serum insulin（INS）, hydrocortisone（HC）, growth hormone（GH） and insulin growth factor 1（TGF-1） levels in dairy cows.【Method】A total of 150 Chinese Holstein cows, weighing（582±41） kg, pregnant with 2-4 fetuses, and fed in a large-scale dairy farm in Sichuan Province from January 2016 to June 2016, were used in the present study. Eighty healthy cows with normal milk and udder that in late pregnancy and gave（15.42±0.71） kg milk per day were chosen. The cows were randomly divided into four groups： groups A, B, C and D, with 20 of them in each group. Stop dairy cows＇ milk by gradually dry milk method and the day when cows gave less milk was recorded as 0 d. Group A was a control group, did not feed compound natural plant preparation and the groups B, C and D were fed 400 g, 500 g, 600 g compound natural plant preparation, respectively, at 8.00 am from 1 d to the day of dry milk completely. Compound natural plant preparation comprises fructus hordei germinatus, mirabilite, rhizoma cimicifugae, bupleurum, rhizoma cyperi, coix seed, silkworm slough, rhizome atratylodis, scutellaria baicalensis, rhizome anemarrhenae, caulis perllae, semen euryales, schisandra chinensis, dandelion, licorice and so on. The cows＇ venous blood was collected from tail on the day 0, 1, 3, 5, 7, 9 and 11, the samples were placed in the centrifuge tubes without anticoagulant at room temperature for 1h. The suspension was centrifuged at 352 × g for 10 min and then put into the refrigerator at-20℃. ELISA was used to evaluate the expression levels of serum INS, HC and IGF-I and the daily milk production was recorded.【Result】Groups A, B, C and D returned milk on the 11 d, 7 d, 5 d and 5 d respectively. The expression levels of serum IGF-1 and HC were declining in all 4 groups and the decline trend of the control group was slower than compound natural plant preparation groups. Th
出处
《中国农业科学》
CAS
CSCD
北大核心
2017年第18期3620-3630,共11页
Scientia Agricultura Sinica
基金
四川省教育厅重点项目(15ZA0024)
四川农业大学双支计划(03571303)
四川农业大学本科科研兴趣培养计划(KY2016062)
关键词
奶牛
回乳
复方天然植物制剂
泌乳激素
dairy cow
milk withdrawal
compound natural plant preparation
galactin
