摘要
筛选并分析生物降解菌Enterobacter sp.在阿特拉津作用下的差异基因。以Enterobacter sp.BIDMC 29基因组为参考,利用高通量测序技术,对阿特拉津降解菌株和对照菌株进行转录组测序分析。结果表明,共有368个基因表现出显著差异,其中上调基因231个,下调基因137个。在差异基因GO富集分析的前30个条目中,尿嘧啶分解代谢、氮利用、硝酸盐同化、硫化氢生物合成、裂解酶活性和过氧化物酶活性等相关基因表现富集。Pathway分析显示,差异基因涉及70条代谢途径,与氮代谢、氨基酸合成和代谢、ABC转运蛋白、丙酮酸代谢等过程有关。菌株通过调节基因表达来提高对阿特拉津的降解能力,为进一步解析阿特拉津的代谢机制奠定基础。
The differential gene of biodegradable bacteria Enterobacter sp. under atrazine stress was screened and analyzed. Transcriptome sequencing of atrazine degrading strain and control strain were analyzed by highthroughput sequencing using Enterobacter sp. BIDMC 29 genome as a reference. There were 368 genes with significant differences, including 231 up-regulated genes and 137 down-regulated genes. Uracil catabolism,nitrogen utilization,nitrate assimilation,hydrogen sulfide biosynthesis,lyase activity,peroxidase activity and other related genes were enriched in top30 of GO analysis. Pathway analysis showed that the differential gene involved 70 metabolic pathways,related to nitrogen metabolism,amino acid synthesis and metabolism,ABC transporter,pyruvate metabolism and other processes. The results showed that degrading strain could improve the degradation ability of atrazine by regulating gene expression and help to further understand the metabolic mechanism of atrazine.
出处
《科学技术与工程》
北大核心
2017年第27期110-115,共6页
Science Technology and Engineering
基金
国家自然科学基金(31400481)
辽宁省博士科研启动项目(20141086)资助
关键词
生物降解
阿特拉津
差异基因表达
污染修复
生物代谢
biodegradation
atrazine
differentially gene expressed
contaminants remediation
biological metabolism
