摘要
目的建立高效液相色谱(HPLC)法测定异甘草酸镁中18β-异构体的含量。方法色谱柱为Agilent Extend-C18(4.6 mm×250 mm,5μm),流动相为0.1 mol·L-1磷酸二氢钾缓冲液(氢氧化钾调pH值至7.0)-乙腈(80∶20),流速1.0 mL·min^(-1),柱温30℃,检测波长250 nm。结果异甘草酸镁与18β-异构体之间的分离度>2.0,18β-异构体的线性范围为0.41~2.46μg·mL^(-1)(r=0.999 8),检测限为0.21 ng,平均回收率为100.2%,99.1%,110.2%,RSD为0.9%,0.1%,0.2%(n=3)。结论该方法操作简便,结果准确,可用于异甘草酸镁中18β-异构体的含量测定。
Objective To establish a HPLC method for determination of 18β-isomer in magnesium isoglycyrrhizinate. Methods The determination was performed by Agilent Extend-C18 column （ 4. 6 mm ×250 mm, 5 μm ） . Mobile phase consisted of 0. 1 mol·L-1 potassium dihydrogen phosphate buffer solution （ adjusted to pH 7. 0 with potassium hydroxide ）-acetonitrile （80︰20） at the flow rate of 1.0 mL·min^-1. The column temperature was 30 ℃, and the detection wavelength was set at 250 nm. Results The resolution of magnesium isoglycyrrhizinate and 18β-isomer was greater than 2.0. The linear range of them was 0.41-2.46μg·mL^-1（ r=0.9998） , the detection limit was 0.21 ng, and the average recovery were 100.2%,99.1%, 110.2%,RSD were 0.9%,0.1%,0.2%（n=3）. Conclusion The method is simple and accurate, and can be used for determination of 18β-isomer in magnesium isoglycyrrhizinate.
出处
《医药导报》
CAS
2017年第10期1179-1182,共4页
Herald of Medicine
基金
国家"重大新药创制"科技重大专项(2008ZX09201-003)
关键词
异甘草酸镁
18β-异构体
色谱法
高效液相
含量测定
Magnesium isoglycyrrhizinate
18β-isomer
Chromatography, high performance liquid
Content determination
