摘要
目的观察药根碱对糖尿病大鼠血管蛋白激酶B/一磷酸腺苷活化蛋白激酶/内皮型一氧化氮合酶(Akt/AMPK/e NOS)信号通路的影响及对糖尿病大鼠可能的保护作用机制。方法将60只雄性Wistar大鼠随机分为正常对照组、模型对照组、药根碱小剂量组和大剂量组,除正常对照组外,其他组通过诱导胰岛素抵抗后空腹腹腔注射链脲佐菌素制作2型糖尿病模型,正常对照组和模型对照组大鼠每日灌胃5%羧甲基纤维素钠溶液,药根碱大剂量组和小剂量组每日分别给予药根碱100,50 mg·kg^(-1),连续8周。检测各组大鼠体质量、血糖及血清胰岛素水平,酶联免疫吸附测定(ELISA)法比较各组大鼠血清中炎症因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平,Western blotting法检测各组大鼠血管中e NOS蛋白及Akt/AMPK通路蛋白的表达。结果与正常对照组比较,模型对照组大鼠体质量下降,血糖升高,血清胰岛素水平显著性升高,差异有统计学意义(P<0.01);与模型对照组比较,药根碱大剂量组体质量显著增加,血糖降低,胰岛素水平下降均差异有统计学意义(P<0.01)。正常对照组血清中IL-1β为(92.3±4.3)pg·mL^(-1),模型对照组为(152.4±20.0)pg·mL^(-1),药根碱小剂量组和大剂量组分别为(120.96±33.0),(95.05±7.7)pg·mL^(-1);模型对照组血清中TNF-α为(10.50±0.82)pg·mL^(-1),正常对照组为(7.48±0.36)pg·mL^(-1),药根碱小剂量组和大剂量组TNF-α分别为(8.82±0.42)和(7.11±0.33)pg·mL^(-1);与正常对照组比较,模型对照组大鼠血管中e NOS表达量及磷酸化的Akt和AMPK含量显著减少,药根碱大剂量组较模型对照组显著提高(P<0.05或P<0.01)。结论药根碱可能通过影响糖尿病大鼠血管中Akt/AMPK/e NOS信号通路及对抗炎症作用对糖尿病大鼠具有保护作用。
Objective To observe the influence of jatrorrhizine on the Akt/AMPK/eNOS signaling pathways and potential protective function in blood vessel of diabetes rats. Methods Male Wistar rats （ n=60） were randomly divided into normal control group, model control group, low-and high dose jatrorrhizine groups. Except normal control group, the other rats were given intraperitoneal injection of STZ after induced insulin resistance, to made typeⅡdiabetes model. CMC-Na solution （5%） was given to normal control and model control group, and the jatrorrhizine resolved in the same solution was administered to low （50 mg·kg^-1） and high dose （100 mg·kg^-1） jatrorrhizine groups for 8 weeks. Their body weight, blood glucose, and seruminsulin levels were measured at the end of the treatment, IL-1β, TNF-αlevel in serum were measured by ELISA, and the eNOS, Akt/AMPK protein expression levels in the blood vessel were measured by Western blotting. Results Compared with normal control group, the weight of model control gropwas lossed, blood glucose was increased（P〈0.01）. Compared with model control group, high-dose jatrorrhizine significantly increased body weight, alleviated blood glucose and decreased serum insulin （ P〈0.01） . Serum inflammatory factor like IL-1βwas （92.3±4.3） pgmL-1 in normal control group, （152.4±20.0） pg·mL^-1 in model control group, （120.96±33.0） pg·mL^-1 and （95.05±7.7） pg·mL^-1 in low-and high-dose jatrorrhizine groups, respectively. TNF-αwas （10.50±0.82） pg·mL^-1 in model control group, （7.48±0.36） pgmL-1 in normal control group, （8.82±0.42） and （7.11±0.33） pg·mL^-1 in low- and high- dose jatrorrhizine groups, respectively. As compared with control group, eNOS and Akt/AMPK expression in blood vessel was significantly reduced （P〈0.05） in model control group, and those were significantly increased in high-dose jatrorrhizine group as compared with model control group （ P〈0.05 or P〈0.01） . Conclusion Jatrorrhizine may exert
出处
《医药导报》
CAS
2017年第10期1107-1111,共5页
Herald of Medicine
关键词
药根碱
糖尿病
抗炎八月
一氧化氮合酶
内皮型
Jatrorrhizine
Diabetes
Anti-inflammation
Nitric oxide synthase,endothelidal
