摘要
目的研究天麻晚期胚胎富集蛋白(LEA2)与抗寒性的关系。方法根据天麻种子转录组测序结果,得到天麻晚期胚胎富集蛋白基因序列,获得重组质粒后导入大肠杆菌进行低温实验。结果获得两条天麻LEA2基因c DNA序列。GeLEA2-1和GeLEA2-2全长分别为477和942 bp,编码158和313个氨基酸,其编码蛋白理论相对分子质量为17.30×103和34.85×103。系统发育分析显示,天麻LEA2基因分别与拟南芥和水稻LEA2基因具有较高相似性。此外,天麻LEA2基因与已知的多种植物的LEA2蛋白具有相似的功能结构域。低温实验显示,与对照组相比,含有GeLEA2重组质粒的大肠杆菌的抗寒性较差。结论推测天麻LEA2为抗寒相关的负向调节基因。
OBJECTIVE To find out the correlation between LEA2 gene from Gastrodia elata and cold resistance. METHODS Two LEA2 cDNA sequences were obtained by screening the transcriptome of seeds from Gastrodia elata. Recombi- nant plasmid was obtained and imported into Escherichia coli. Then the transformed Escherichia coli（pET28a-LEA2） received low temperature treatment. RESULTS GeLEA2-1 had a complete 477 bp ORF encoding 158 amino acids and its molecular weight was 17.3 × 103. In addition, GeLEA2-2 had a complete 942 bp ORF encoding 313 amino acids and its molecular weight was 34.85 × 103. Phylogenetic analysis showed the two GeLEA2 were highly homologous with LEA2 from Arabidopsis thaliana and Oryza sativa. The growth curves of E. coli BL21 （ DE3 ） showed that the strain expressing GeLEA2 tolerated low temperature more poorly than the control. CONCLUSION Our work reveals that GeLEA2 could response to low temperature stress by negative regulation.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2017年第19期1664-1669,共6页
Chinese Pharmaceutical Journal
基金
中国医学科学院基本科研业务项目(2016ZX350062)
