摘要
以磺胺二甲氧嘧啶钠(SDM)作为主要研究的磺胺类抗生素,观察其在猪粪混合发酵液中的代谢过程,以及对厌氧发酵过程中产气量与酶活性的影响。发酵过程共设置4个浓度处理:CK处理、T1处理(SDM添加量为30 mg/kg)、T2处理(SDM添加量为60mg/kg)、T3处理(SDM添加量为120 mg/kg)。结果表明,厌氧发酵过程中SDM对尿酶活性呈现出"抑制-激活-抑制"的作用。在一定添加浓度范围内(T1≤T3),磺胺二甲氧嘧啶钠对厌氧过程中脱氢酶的活性有一定的促进作用。SDM在混合发酵液中降解遵从一级动力学规律,代谢速率为T2>T1>T3,而T3处理对COD去除率达最大为48%,其累积产气量是空白处理累积产气量的2倍。随着磺胺二甲氧嘧啶钠添加量的增加促进了更多的产酸菌群的生长,合成更多的甲烷形成的前驱体,以得到更多的累积产气量。
The degradation rate of SDM （sulfadimethoxine sodium）, the variation of methane production and enzyme activities in pig manure spiked with different concentrations of SDM were studied during anaerobic fermentation process. There are four treatments for SDM, including CK （0 mg/kg）, T1 （30 mg/kg）, T2 （60 mg/kg） and T3 （120 mg/kg）. The results showed that a similar inhibition of urease activities were measured at the beginning and at the end of the incubation period but not at the intermediate time. The dehydrogenase activities inhibition was the highest at lowest level and decreased with increasing concentration of SDM （T1≤T3）. The degradation of SDM in anaerobically digested effluent obey the first order kinetics （T2 〉T1 〉T3）. At the end of fermentation, SDM enhanced the biogas production about two-fold compared to the control. Adding SDM fostered the growth of acid-producing bacterial communities, which synthesized more precursors for methane formation.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2017年第8期18-24,共7页
Environmental Science & Technology
基金
湖北省重大科技创新计划项目(2014A010)
中南民族大学校基金(CZW15098)
关键词
猪粪
磺胺二甲氧嘧啶钠
厌氧发酵
酶活性
微生物菌群
manure
sulfadimethoxine sodium
anaerobic digestion
enzymatic activity
microbial community
