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HPLC法测定补肾强筋胶囊中3种成分的含量 被引量:1

Determination of Three Components in Bushenqiangjin Capsules by HPLC
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摘要 目的建立测定补肾强筋胶囊中淫羊藿苷、柚皮苷及松脂醇二葡萄糖苷含量的HPLC法。方法色谱柱为SHIADZU VP-ODS(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速:1.0 ml·min-1,柱温:30℃,检测波长:277nm。结果淫羊藿苷、柚皮苷、松脂醇二葡萄糖苷分别在4.2~104.4、1.7~42.5、5.5~137.5μg·ml-1范围内与峰面积线性关系良好(r=0.9999);平均回收率分别为98.65%(RSD为1.4%)、98.47%(RSD为1.8%)、97.96%(RSD为1.6%)。结论所建方法可用于补肾强筋胶囊3种成分的含量测定。 Objective To establish an HPLC method for simultaneous determination of the contents of icari- ine,naringin and pinoresinol diglucoside in Bushenqiangjin capsules.Methods Chromatographic separation was car- ried out on a SHIADZU VP-ODS column(250 mm× 4.6 mm,5 μm)with a mobile phase consisting of acetonitrile and water in a gradient elution mode. The flow rate was 1.0 ml o min-1.The detection wavelength was 277 nm, and the column temperature was 30℃.Results The linear range of icariine, naringin and pinoresinol diglucoside was 4.2- 104.4,1.7--42.5 and 5.5-- 137.5 μg ml- 1 respectively.The average recovery was 98.65 % (RSD was 1.4% ), 98.47% (RSD was 1.8%),and 97.96%(RSD was 1.6 % ) respectively.Conclusion This established standard is appli cable to content determination of Bushenqiangjin capsules.
作者 严倩茹
出处 《解放军药学学报》 CAS CSCD 2017年第4期348-349,352,共3页 Pharmaceutical Journal of Chinese People's Liberation Army
关键词 补肾强筋胶囊 淫羊藿苷 柚皮苷 松脂醇二葡萄糖苷 HPLC Bushenqiangjin capsules icariine naringin pinoresinol diglucoside HPLC
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