摘要
目的:利用天然高分子材料明胶、海藻酸钠的物理、生物学性能及其特殊的交联特性,构建一种可用于3D生物打印的生物墨水,并对其进行制备、表征与分析,为后期的生物打印提供材料基础。方法:根据回顾文献得到的信息,采用比例混合法制备质量比为2∶15、2∶10的2种海藻酸钠/明胶复合水凝胶,分别进行冻干扫描电镜(SEM)微观形貌、溶胀性能、孔隙率分析,3D打印以及利用复合水凝胶进行载ATDC-5小鼠成软骨前体细胞的3D培养等实验,以确定此复合水凝胶的物理特性及生物学特性。结果:顺利构建出海藻酸/明胶复合水凝胶,并对其进行分析,发现2∶10、-20℃组凝胶比2∶15、-50℃组水凝胶拥有更多、更大的孔径。孔隙率实验结果显示,2组海藻酸钠/明胶复合水凝胶的孔隙率在60%~82%之间,均利于细胞培养。溶胀性能测试结果显示,溶胀率均在660%~740%范围,表明此种海藻酸钠/明胶复合水凝胶的亲水性较强,非常有利于细胞增殖。3D打印结果显示,利用此种复合水凝胶可以打印出预先设计的几何形状,且经二价阳离子化学交联后,能够形成具有一定机械强度的水凝胶胶体。细胞培养结果显示,ATDC-5细胞在此种复合水凝胶内培养下,7 d后仍有95%的成活率。结论:此种经明胶改性的海藻酸钠/明胶复合水凝胶具有优良的微观形貌、溶胀性能以及适合的孔隙率,并且具备3D打印能力。尤其是明胶与海藻酸钠质量比为15∶2组,因具有均匀孔径以及能够满足3D打印墨水的性能要求,可考虑作为3D生物打印的"墨水"。
PURPOSE： Based on the physical and biological properties of natural polymer gelatin and sodium alginate and their special crosslinking characteristics, a bio-printing gel was built that can be used for 3D bio-printing. METHODS： Based on review of the literature, two kinds of sodium alginate/gelatin composite hydrogels were produced, whose mass ratio was 2：10 and 2：15, respectively. The hydrogels were studied by scanning electronic microscopic （SEM） microstructure, swelling properties and porosity via freezing-drying method. Then, the hydrogels were used for culturing ATDC-5 cells and 3D printing. RESULTS： The alginate/gelatin composite hydrogel was successfully constructed and a lump of special 3D geometrical shaped gel was constructed. The experimental results showed that 2：10, -20℃ group had more larger aperture than 2：15,-50℃ group. This kind of alginate/gelatin composite hydrogel had porosity between 60% and 82%. The swelling ratio was in the range of 660% to 740%. The composite hydrogel could be used for 3D printing and constructing certain mechanical strength of gel via bivalent cation chemical crosslinking. The mean cell viability was 95%±2.3% by 3D cell encapsulation culture for 1,3,7 days. CONCLUSIONS： This modified alginate/gelatin composite hydrogel could be used as 3D bio-printing ink and had a good application prospect, especially when the gelatin/alginate ratio was 15：2.
出处
《中国口腔颌面外科杂志》
CAS
2017年第5期402-407,共6页
China Journal of Oral and Maxillofacial Surgery
基金
上海交通大学医工交叉项目(YG2015QN22)
