摘要
目的:通过检测MCM7和HPV在上颌窦鳞癌(squamous cell carcinoma of the maxillary sinus,MSSCC)中的表达,探讨其在MSSCC发病机制中的作用及临床意义。方法:采用免疫组织化学方法检测MSSCC(实验组)、癌旁组织(癌旁组)和下鼻甲黏膜组织(对照组)中的MCM7及HPV的表达,应用SPSS 22.0软件包对数据进行统计学分析。结果:(1)MCM7在癌旁组和对照组上皮中大部分位于基底层,而其在MSSCC中呈弥散分布。MCM7在对照组(P=0.000)、癌旁组(P=0.000)和实验组(P=0.000)中的表达水平逐渐增高。(2)MCM7的表达仅与肿瘤分化程度有关(P=0.030),并且随着分化程度的下降,MCM7的表达水平逐渐增高。(3)伴HPV感染的细胞无论在对照组、癌旁组或实验组中分布均无明显规律。HPV在对照组、癌旁组及实验组患者中的感染率分别为40.00%(4/10)、95.65%(22/23)和78.57%(33/42)。(4)HPV感染与颈淋巴结转移有关(P=0.015),而与患者性别(P=1.000)、年龄(P=0.332)、吸烟(P=0.536)、临床分期(P=0.806)及肿瘤分化程度(P=0.489)无关。(5)在实验组中,伴HPV感染者,MCM7表达水平显著高于未感染者(P=0.007),且呈正相关关系(rs=0.317,P=0.041)。结论:MCM7和HPV可能与MSSCC的发生、发展有关,可能对患者的临床治疗及预后判断具有重要指导意义;MCM7异常表达可作为筛查HPV感染病例的良好临床病理学指标。
PURPOSE： This study was designed to investigate the expression of minichromosome maintenance protein 7 （MCM7） and human papillomavirus （HPV） in squamous cell carcinoma of the maxillary sinus （MSSCC） and its relationship with the pathogenesis of this disease and clinical significance. METHODS： Using immunohistoehemistry method, we studied the expression of MCM7 and HPV in MSSCC （experimental group）, corresponding adjacent tissues （adjacent group） and inferior turbinate mucosal tissues （control group）. SPSS 22.0 software package was used to analyze the data. RESULTS： MCM7 was located in the basal layer of the epithelium in the adjacent group and the control group, but diffusedly distributed in the MSSCC. The expression of MCM7 in control （P=0.000）, adjacent （P=0.000） and experimental （P=0.000） group increased gradually. The expression of MCM7 was only related to the degree of tumor differentiation （P= 0.030）, and the expression of MCM7 increased gradually with the decrease of the degree of differentiation. The cells infected with HPV had no obvious regularity in the control, the adjacent or the experimental group. The infection rates of HPV in the control group, the adjacent group and the experimental group were 40.00% （4/10）,95.65% （22/23）38.57% （33/ 42）, respectively. HPV infection was associated with cervical lymph node metastasis （P=0.015）, but not gender （P=1.000）, age （P=0.332）, smoking （P=0.536）, clinical stage （P=0.806） and tumor differentiation （P=0.489）. The expression level of MCM7 in patients with HPV infection was significantly higher than that in the uninfected patients （P=0.007）, and there was a positive correlation between them （rs=0.317, P=0.041）. CONCLUSIONS： MCM7 and HPV maybe correlate with the occurrence and development of MSSCC, which may have important significance for clinical treatment and prediction of prognosis.
出处
《中国口腔颌面外科杂志》
CAS
2017年第5期392-396,共5页
China Journal of Oral and Maxillofacial Surgery
基金
辽宁省科技厅科学技术计划项目(2014225020)
关键词
上颌窦
鳞状细胞癌
微小染色体维持蛋白7
人乳头瘤病毒
Maxillary sinus
Squamous cell carcinoma
Minichromosome maintenance protein 7
Human papillomavirus
