摘要
目的观察COX-2、bcl-2及CD31在结直肠癌、癌旁组织及腺瘤中的表达情况,并探讨COX-2和bcl-2相关性及二者与CD31表达计数的关系。方法运用免疫组织化学法检测38例结直肠癌组织、38例癌旁组织及29例癌前病变中COX-2、bcl-2及CD31表达情况。结果 (1)bcl-2的表达与结直肠癌患者年龄有显著相关性(P<0.05),COX-2的表达与患者年龄和癌灶大小无关(P>0.05);(2)COX-2与bcl-2表达在结直肠癌、癌旁组织及腺瘤三组中两两比较有显著性差异,癌组内较高(P<0.05);(3)COX-2表达在癌高中分化组与低分化组有显著性差异(P<0.05),bcl-2表达在高分化组与中低分化组有显著性差异(P<0.05);且MVD计数在淋巴结转移与非转移组有显著差异(P<0.05);(4)COX-2与bcl-2高表达组与低表达组中,MVD计数均有统计学意义(P<0.05);(5)在癌组COX-2与bcl-2表达无明显相关性(P>0.05),但COX-2与bcl-2高中表达组的MVD计数呈正相关性(P<0.01)。结论结直肠癌中COX-2与bcl-2表达提高能促进CD31表达即小血管增生及淋巴结转移,联合检测有可能作为筛查评估结直肠癌的重要手段。
Objective To investigate the expression of COX-2, bcl-2 and CD31 in colorectal carcinoma, adjacent tissues and adenoma, and to explore their relationships. Methods Im- munohistochemistry was used to detect the expression of COX-2, bcl-2 and CD31 in 38 cases of colorectal cancer tissues, 38 cases of carcinoma adjacent tissues and 29 cases of cancer adeno- ma. Results ①the expression of bcl-2 was significantly correlated with the age of patients with colorectal cancer （ P 〈 0. 05 ）. The expression of COX-2 was not related to the age and the size of the tumor （ P 〉 0. 05）.②the expression of COX-2 and bcl-2 in colorectal cancer, para- cancerous tissues and adenomas were significantly different between the three groups （P 〈 0. 05 ）, and the cancer group was higher in the group. ③the expression of COX-2 in high dif- ferentiated group had significant difference with low differentiation group （ P 〈 0. 05 ）, the ex-pression of bel-2 in high differentiation group had significant difference with low differentiation group （P 〈 0. 05 ） ; and the expression of MVD in lymph node metastasis and non metastasis group was significant difference （ P 〈 0. 05 ）. ④COX-2 and bcl-2 high expression group and low expression group, MVD were significantly different （ P 〈 0. 05 ）. ⑤COX-2 was positively correlated with MVD in the high and middle bcl-2 group （P 〈 0. 01 ） , but there was no signifi- cant correlation between the expression of COX-2 and bcl-2 in the cancer group （ P 〉 0. 01 ）. Conclusion The expression of COX-2 and bcl-2 was increased in colorectal cancer, and pro- motes angiogenesis and lymph node metastasis, that there are associated with each other, and joint detection may be used as an important means of screening for colorectal cancer.
出处
《哈尔滨医科大学学报》
CAS
2017年第4期321-325,共5页
Journal of Harbin Medical University
