摘要
目的:探讨前交叉韧带(anterior cruciate ligament,ACL)离断联合半月板切除诱发的骨关节炎(osteoarthritis,OA)的基因表达和生物过程的改变,为OA分子机制的进一步研究提供生物信息学依据。方法:从GEO数据库下载Appleton等构建的ACL离断联合半月板切除诱发的OA早期大鼠模型的基因芯片数据集,应用生物信息学方法筛选差异表达基因(differentially expressed genes,DEGs),并进行基因本体论(Gene ontology,GO)和日本京都基因和基因组百科全书代谢通路数据库(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。结果:共筛选出170个DEGs,其中包括97个上调基因和73个下调基因。上调基因主要富集在细胞外基质,并与细胞外基质交互通路等信号通路关系密切。而下调基因主要富集在肌肉收缩的生物学功能中,并与PPAR通路等信号通路关系密切。结论:细胞外基质和肌肉收缩的改变对OA的发生发展起着关键作用。细胞外基质受体交互通路和PPAR信号通路与OA密切相关,值得进一步深入研究。
Objective To explore the changes in gene expression and biological process of the osteoarthritis （OA） induced by anterior cruciate ligament （ACL） transection and partial medial meniscectomy,so as to provide bioinformatic basis for further studying the molecular mechanism of OA.Methods The gene chip datasets of a rat model of early 0A induced by ACL transection and partial medial meniscectomy were downloaded from GEO databases （submitted by Appleton,et al.）.The differential expression genes （DEGs）were identified,and the Gene ontology （GO） as well as the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analyses for DEGs were conducted using bioinformatic methods.Results A total of 170 DEGs including 97 up-regulated genes and 73 down-regulated genes were identified.The up-regulated genes were mainly enriched in the extracellular matrix （ECM） and were closely related to the ECM-receptor interaction,while the down-regulated genes were mainly enriched in the biological function of muscle contraction and were linked with the peroxisome proliferators-activated receptor （PPAR） signaling pathway.Conclusion The changes of ECM and muscle contraction play a key role in the occurrence and development of OA.The ECM-receptor interaction and PPAR signaling pathway are strongly associated with OA and worthy of further study.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2017年第9期788-792,787,共6页
Chinese Journal of Sports Medicine
基金
国家高技术研究发展计划(863计划)(2015AA033703)
国家自然科学基金(81572108
81370052)
国家重点研发计划项目(2016YFC1100300)
关键词
骨关节炎
差异表达基因
生物信息学
osteoarthritis,differentially expressed genes, bioinformatics
