摘要
目的研究大气污染颗粒(PM2.5)对博莱霉素诱导肺纤维化的影响及分子机制。方法 24只雌性C57BL/6小鼠随机分为假手术组、博莱霉素组和PM2.5组,其中博莱霉素组和PM2.5组小鼠气管一次性滴注博莱霉素5 mg/kg,假手术组滴注等体积生理盐水。造模后第1天气管内给药,PM2.5组和博莱霉素组分别滴注100 mg/kg PM2.5和等体积生理盐水。21 d后处死小鼠,HE和Masson染色观察肺组织病理变化,碱水解法测定肺组织羟脯氨酸含量,免疫印迹检测肺组织中IRE1和CHOP蛋白表达量。结果与假手术组相比,博莱霉素组发生明显肺炎和肺纤维化,羟脯氨酸含量明显升高(P<0.01),与博莱霉素组比较,PM2.5能加重肺纤维化程度,增加羟脯氨酸含量,促进IRE1和CHOP蛋白表达(P<0.01)。结论 PM2.5加重博莱霉素诱导的小鼠肺纤维化可能涉及内质网应激通路。
Objective To investigate the effect and molecular mechanism of air pollution particle PM2.5 on bleomycin- induced pulmonary fibrosis in mice. Methods Twenty-four female C57BL/6 mice were randomly divided into sham, bleomycin and PM2.5 groups. Bleomycin and PM2.5 groups were intratracheally injected with 5 mg/kg bleomycin, while sham group were given with normal saline. Next day, PM2.5 and bleomycin groups received intratracheal injection of 100 mg/kg PM2.5 and normal saline, respectively. The mice were sacrificed after 21 days, and pulmonary pathology, hydroxyproline content, and IRE1 and CHOP expression were detected by HE and Masson stain, alkaline hydrolysis method, and Western blot, respectively. Results The severity of pneumonia and pulmonary fibrosis and level of hydroxyproline were higher in bleomycin group than in sham group （P〈0.01）. Compared with bleomycin group, pulmonary fibrosis, hydroxypro- line content, and IRE1 and CHOP expression were increased in PM2.5 group （P〈0.01）. Conclusion PM2.5 may exacerbate the bleomycin-induced pulmonary fibrosis through endoplasmic reticulum stress pathway in mice.
出处
《广东医科大学学报》
2017年第3期237-240,共4页
Journal of Guangdong Medical University
基金
国家自然科学基金项目(No.81172615)
