摘要
目的探讨应用紫外线照射未成熟树突状细胞诱导C3H小鼠对Balb/c小鼠同种抗原免疫耐受及其免疫学机制。方法 (1)用紫外线B(ultraviolet B,UVB)照射Balb/c(H-2d)小鼠骨髓来源的未成熟树突状细胞(immature dendritic cells,im DC),诱导其凋亡,随后将其输注到C3H(H-2k)小鼠体内,诱导C3H小鼠对Balb/c抗原免疫耐受。(2)检测诱免疫耐受小鼠体内调节T细胞以及细胞因子白细胞介素-10(interleukin-10,IL-10)和干扰素-γ(interferon-γ,IFN-γ)分泌以研究其耐受机制。结果 (1)UVB-Balb/c im DC免疫的C3H小鼠对Balb/c抗原产生了免疫耐受,不能产生抗Balb/c抗体。(2)免疫耐受C3H小鼠不能排异注射到体内的Balb/c脾细胞。(3)UVB-Balb/c im DC免疫的C3H小鼠T细胞分泌IL-10增加,并且产生更多的FOX-P3+调节T细胞。结论 (1)应用UVB-Balb/c im DC免疫C3H小鼠可以使C3H小鼠对Balb/c抗原产生完全彻底的免疫耐受。(2)免疫耐受C3H小鼠对Balb/c抗原产生免疫耐受的可能原因是由于其T细胞分泌IL-10增加,并且产生更多的FOX-P3^+调节T细胞。
Objective To induce alloantigen immune tolerance between C3H mice and Balb/c mice by infusing the ultraviolet B（UVB） radiation immature dendritic cells （imDC） and and study the immunological mechanisms of this process.Methods ①The authors induced alloantigen tolerance in C3H mice （H-2k） by intravenous injecting ultraviolet B （UVB） irradiated Balb/c immature dendritic cells （UVB-Balb/c imDC） derived from the cultures of Balb/c bone marrow cells. ②Detection of immune tolerance induced regulatory T cells, interleukin-10 （IL-10） and interferon-γ （IFN-γ） in mice in order to study the tolerance mechanism. Results ①C3H mice immunized with UVB-Balb/c imDC can produce immune tolerance to Balb/c antigens and can not produce anti-Balb/c antibody. ②C3H mice immunized with UVB-Balb/c imDC can not clear the Balb/c spleen cells in vivo. ③The T cells of C3H mice immunized with UVB-Balb/c imDC can secrete more IL-10 and produce more FOX-P3＋ regulatory T cells than the control group in vitro. Conclusion ①Immunization of C3H mice with UVB-Balb/c imDC allows C3H mice to fully tolerate Balb/c antigen. ②The possible cause of immune tolerance to Balb/c antigens in C3H mice is due to the increased secretion of IL-10 from T cells and the production of more FOX-P3＋regulatory T cells.
出处
《首都医科大学学报》
CAS
北大核心
2017年第3期431-438,共8页
Journal of Capital Medical University
基金
国家自然科学基金(81172854
81240015)~~
关键词
树突细胞
凋亡
调节T细胞
免疫耐受
dendritic cells apoptosis regulatory T cells immune tolerance
