摘要
目的考察九节龙皂苷对大鼠及小鼠不同状态下胃肠功能的兴奋作用。方法给予正常小鼠及阿托品导致胃肠功能障碍小鼠九节龙皂苷后测定其胃排空及肠推进,分别测定在正常克氏液和低钙克氏液中孵育的大鼠离体小肠收缩性,并用Western blot法检测九节龙皂苷对便秘大鼠肠肌球蛋白轻链激酶(MLCK)表达的影响。结果九节龙皂苷可促进正常及胃肠功能障碍小鼠的胃排空及肠推进,增强大鼠离体小肠收缩性,且可刺激便秘大鼠MLCK的表达。结论九节龙皂苷可促进平滑肌MLCK的表达,进而增强胃肠平滑肌运动功能。
Objective To investigate the stimulating effects of ardipusilloside - 1 （ARD - I ） on gastro - intestinal function. Methods The gastric emptying and intestinal propulsion in normal mice or gastro - intestinal dysfunction mice induced by atropine were determined respectively, the contractibility of isolated intestine in rats was researched, and the expression of myosin light chain kinase （MLCK） in intestine in rats was detected by Western blot method. Results ARD - I could promote the gastric emptying and intestinal propulsion in mice in any state, improve the contractibility of isolated intestine in rats incubated in normal krebs solution or low calcium krebs solution, and enhance the expression of MLCK in intestine in rats. Conclusion ARD - I showed stimulating effects obviously on gastro - intestinal function, which might be reached by increasing the expression of smooth muscle MLCK.
作者
徐志立
陶小军
张明波
窦德强
康廷国
Xu Zhili Tao Xiaojun Zhang Mingbo et al(College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Liaoning 116600, China)
出处
《医学研究杂志》
2017年第9期42-45,共4页
Journal of Medical Research
基金
中国博士后科学基金资助项目(2012M510834)
关键词
九节龙皂苷
胃肠功能
肌球蛋白轻链激酶
Ardipusilloside - I （ARD - I ）
Gastro -intestinal function
Myosin light chain kinase （MLCK）
