摘要
目的研究吴茱萸碱(Evo)对人结肠癌荷瘤Balb/c裸鼠HCT-116细胞增殖的影响,并探讨其可能机制。方法用HCT-116细胞接种到4周龄的Balb/c裸小鼠右下腋部,待荷瘤直径约0.5 cm后用灌胃针灌入Evo(3 mg/kg)进行治疗,每3 d测瘤体直径及小鼠质量,绘制质量曲线及瘤体体积曲线,灌喂22 d后处死,取瘤体组织;HE染色法验证Balb/c裸鼠瘤体的成瘤情况;免疫组化检测瘤体HDAC3、NF-κB、p53的表达;Westernblot检测瘤体组蛋白去乙酰化酶HDAC3、NF-κB、p53的变化。结果 Evo灌喂组小鼠的瘤体体积和瘤体质量明显小于对照组,质量较对照组重;Evo灌喂组肿瘤细胞皱缩,胞核深染,较对照组核分裂像明显减少;经吴茱萸碱处理的裸鼠的瘤体中NF-κB、p53表达量较对照组增高,HDAC3则反之(P<0.05)。结论吴茱萸碱可以通过下调HDAC3来影响NF-κB及p53蛋白的表达,抑制人结肠细胞系HCT-116的体内增殖。
Objective To investigate the effect of evodiamine on proliferation of HCT-116 in balb/c nude mice,and to explore possible mechanism. Methods HCT-116 cells were injected into the right armpit of 4 week old Balb/c nude mice,the feeding had been executed at the time of the diameter of the xenografted tumor reached0. 5 cm,at the dose of 3 mg/kg（ Evo）,body weight and tumor diameter had been tested every three days,and made the curve of body weight and tumor diameter of the mice. All the mice were sacrificed after 22 days of feeding,and harvested the xenografted tumors. The morphological difference of the two groups tumor were identified by HE staining,the expression of HDAC3,NF-κB and p53 protein were detected by IHC and Western blot. Results Compared with the control groups,the volume and weight of the tumors in Evo groups were significantly lighter,and the body weight of the nude mice were heavier,the tumor cells in Evo groups were shrink,deeply staining nucelus and their abnormal mitoses were fewer,the expression of NF-κB and p53 were increased but HDAC3 was decreased in xenografted tumors treated with Evo（ P〈0. 05）. Conclusions Evo can change the expression of NF-κB and p53 by down-regulating HDAC3,and inhibit the proliferation of HCT-116 cells line in vivo.
作者
石雪萍
李晓朋
熊伟
李海星
郭珮
王芬
陈地龙
李静
SHI Xue-ping LI Xiao-peng XIONG Wei LI Hai-xing GUO Pei WANG Fen CI-IEN Di-long LI Jing(Dept. of Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China)
出处
《基础医学与临床》
CSCD
2017年第10期1373-1377,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(31271368)
