摘要
为了解川芎(Ligusticum chuanxiong Hort.)阿魏酸生物合成相关基因的密码子使用偏好性特点,为运用基因工程技术实现阿魏酸的异源生物合成提供理论依据,对川芎转录组中共50 108条Unigenes使用Codon W、Cusp和Chips进行在线分析。结果表明,总GC含量为41.4%,有效密码子占总数的16.17%,最优密码子偏好以A/U结尾,表明川芎转录组Unigenes密码子偏好程度整体水平不高。比较分析了川芎转录组中阿魏酸生物合成相关基因(PAL、C4H、C3H与COMT)与不同模式生物的稀有密码子,表明与大肠杆菌基因组密码子使用频率差值较大的有4个,与酵母、烟草和拟南芥基因组差值较大的均有3个,这预示着川芎阿魏酸生物合成相关基因在酵母、烟草和拟南芥中的表达效率较高。
In order to understand the codon usage preference of genes involved in the biosynthesis of ferulic acid in Ligusticum chuanxiong Hort.,and thereby providing a theoretical basis for the application of genetic engineering technology to achieve heterologous biosynthesis of ferulic acid,50 108 unigenes of L. chuanxiong transcriptome were analyzed on-line using Codon W,Cusp and Chips at first. As a result,the total GC content was 41.4%,valid codons was 16.17% and the optimal codon preferred A/U as end. Furthermore,the rare codons of PAL,C4 H,C3H and COMT were compared with those of candidate expressed hosts,including E. coli,yeast,tobacco and Arabidopsis thaliana. As a result,4 codons were found between E.coli and L. chuanxiong,and 3 codons were found among yeast,tobacco and Arabidopsis thaliana. It suggests high expressional ratio of PAL,C4 H,C3H and COMT might take place in yeast,tobacco and Arabidopsis thaliana.
作者
李慧娟
潘思皓
杜函圳
孙静莹
刘蓄坚
谭艳
周嘉裕
廖海
LI Hui-juan PAN Si-hao DU Han-zhen SUN Jing-ying LIU Xu-jian TAN Yan ZHOU Jia-yu LIAO Hai(School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China Chengdu Military General Hospital,Chengdu 610083,China)
出处
《湖北农业科学》
2017年第18期3549-3553,共5页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(31500276)
四川省应用基础研究项目(2017JY0222)
成都市科技技术研发项目(2015-HM01-00051-SF)
省级大学生创新创业训练计划资助项目(2016088)
中央高校基本科研业务费医学类专题研究项目(NO.2682016YXZT09)
