摘要
目的探讨三伏灸不同灸量影响哮喘型新西兰兔气道重塑的作用机理。方法选取30只雄性新西兰兔,随机分为空白组、模型组、对照组、实验组、地塞米松组。支气管哮喘气道重塑模型造模后,于"三伏天"以隔姜灸加中药贴敷法干预。实验结束后,取新西兰兔肺组织,检测肺组织MMP-9的蛋白表达及MMP-9基因表达。结果与空白组比较,模型组、对照组、实验组、地塞米松组MMP-9蛋白及基因表达明显增高(P<0.05);与模型组比较,实验组、对照组、地塞米松组的MMP-9蛋白及基因表达明显降低(P<0.05);实验组与地塞米松组的MMP-9蛋白及基因表达无统计学差异(P>0.05)。结论增加三伏隔姜灸量达到9壮可以更好地通过抑制MMP-9的合成,改善支气管哮喘型新西兰兔气道重塑。
Objective To observe the effect of MMP-9 expression about NewZealand white rabbits which treat by different amounts of dog days moxibustion. Methods 30 male NewZealand white rabbits were randomly divided into blank group, model group, control group, experimental group and dexamethasone group. Airway remodeling of asthma model, experimental group and the control group were given medicine applicator Ginger joint intervention groups Ginger and sticking points were selecting Dazhui and bilateral Feishu. At the end of the experiment, rabbit＇s lung tissue was taken, and the protein expression of MMP-9 and MMP-9 gene expression were detected. Results Comparing with the blank group, in the model group, control group, experimental group and dexamethasone group, the protein and gene expression of MMP-9 were significantly increased（P0.05）. Comparing with the model group, the protein and gene expression of MMP-9 were significantly decreased in the control group, experimental group and dexamethasone group（ P〈0.05）. There was no significant difference in protein and gene expression of MMP-9 between the experimental group and the dexamethasone group（ P〈0.05）. Conclusion Ginger moxibustion reached 9 moxa-cones can improve positive changes of bronchial asthma type rabbit bronchial morphological structure, so as to improve the bronchial asthma symptoms of airway remodeling in NewZealand rabbits, and effectively alleviate the symptoms of bronchial asthma.
出处
《中国中医药现代远程教育》
2017年第17期143-145,共3页
Chinese Medicine Modern Distance Education of China
基金
国家自然科学基金项目【No.81403490】
福建省属公益类科研院所基本科研专项资助项目【No.2015R1035-3】
福建省卫计委中青年骨干人才培养项目【No.2014-ZQN-JC-33】
关键词
三伏灸
哮喘
MMP-9
气道重塑
动物实验
新西兰兔
Dog-day moxibustion
asthma
MMP-9
airway remodeling
animal experiment
New Zealand rabbits
