摘要
目的研究猪阔筋膜细胞外基质的生物力学性能及相容性，探讨开发一种新的组织修复材料。方法将新鲜猪阔筋膜和人阔筋膜经脱细胞方法处理，制成细胞外基质，对正常猪阔筋膜、猪阔筋膜细胞外基质和正常人阔筋膜细胞外基质进行组织形态学和生物力学性能检测，比较三者之间的差异。将新西兰大白兔背部制成损伤模型，分别用猪阔筋膜细胞外基质、正常猪阔筋膜及异体兔阔筋膜进行修补，记录术后不同时段切口情况并取出置入阔筋膜行HE染色，观察组织结构，比较组织相容性。结果经脱细胞处理的猪阔筋膜细胞成分去除完全，胶原纤维排列较疏松，孔隙大，呈丝网状；脱细胞猪阔筋膜与正常猪阔筋膜及正常人阔筋膜极限抗张强度分别为（4．47±0．54）MPa、（4．49±0．91）MPa、（4．79±1．35）MPa，三者比较差异无统计学意义（P〉0．05）；脱细胞猪阔筋膜细胞外基质与正常猪阔筋膜断裂伸长率分别为501．83％±52．01％、661．17％±200．28％，差异无统计学意义（P〉0．05），两者与正常人阔筋膜细胞外基质（98．08％±26．02％）比较，断裂伸长率差异有统计学意义（P〈0．01）。HE染色显示，置入兔背部后，猪阔筋膜细胞外基质的炎性反应明显轻于正常猪阔筋膜。结论猪阔筋膜细胞外基质具有良好的生物力学性能及生物相容性。
Objective To study the biomechanieal property and histoeompatibility of acellular porcine fascia so as to supply a new substitute material for tissue repair. Methods Samples of normal porcine fascia, acellular porcine fascia and normal human fascia were prepared for histological and biomechanical examination. Xenogeneic fresh porcine fascia, acellular porcine fascia and allogeneic rabbit fascia were implanted into the back of rabbit. Tissues were taken for HE staining and histocompatibility test. Results Histological examination showed that the cellular components which elicit immune rejections had been removed in the acellular porcine fasciam, with the complete extracellular matrix reserved. Arrangement of collagen fiber was loose in the acellular porcine fascia. The biomechanical performance test of the three samples showed that there was no significant difference in the extreme tensile strength （4.47 ± 0.54） MPa, （4.49 ± 0.91 ） MPa, （4.79 ± 1.35 ） MPa for acellular porcine fascia, normal porcine fascia and normal human fascia respecively, P 〉 0.05 ）. There was no significant difference between acellular porcine fascia extracellular matrix and porcine fascia with the cell in breaking elongation （501.83% ± 52.01% ,661.17% ±200.28% ,P 〉 0. 05）. However, there was a significant difference in the breaking elongation between human fascia （98.08% ± 26.02% ） and the other 2 kinds of porcine fascia （ P 〈 0.01 ）. Histocompatibility test showed the local reactions of the acellular porcine fascia group were weaker than the normal porcine fascia group. Conclusions With sound preparativemethod, the acellular porcine fascia has good biomechanical property and histocompatibility and may serve as a good alternative material for tissue repair.
出处
《中华整形外科杂志》
CSCD
北大核心
2017年第5期373-378,共6页
Chinese Journal of Plastic Surgery
基金
山东省医药卫生科技发展计划项目（2013WS0224）
关键词
阔筋膜
细胞外基质
猪
生物力学
组织相容性
Fascia lata
Extracellular matrix
Swine
Biomechanics
Histocompatibility
