摘要
鱼类能量学研究通常用耗氧率(MO_2)表征其某一生理状态的代谢强度.为考察不同流量和时间对鱼类标准代谢率(SMR)测定的影响,本研究在水温(25±0.5)℃条件下选取80尾鱼体健康、体质量相近的中华倒刺鲃Spinibarbus sinensis幼鱼作为实验鱼,研究在不同流量和测定时间条件下中华倒刺鲃幼鱼SMR数据的变化规律及稳定性.实验共设定5个流量梯度(1L/h,2L/h,3L/h,4L/h,5L/h),每一流量连续测量6个时间点,每隔1h测定1次SMR.结果显示:随着流量(v)逐渐加快,实验鱼呼吸室与空白呼吸室的溶氧差值(ΔMO_2)呈指数型下降,二者的拟合方程为ΔMO_2=0.872 2 v^(-0.999 8)(R^2=0.994,p<0.001).不同流量条件下,中华倒刺鲃幼鱼ΔMO_2的变异系数差异明显(p<0.001);其中,在流量5L/h条件下,ΔMO_2的变异系数最大.设定流量对中华倒刺鲃幼鱼SMR测定无显著影响(p>0.05).当流量越大时,在不同测定时间下SMR重复性的比例趋于变小.在设定的流量范围内中华倒刺鲃幼鱼SMR无显著差异,考虑样本量的大小、实验鱼体质量及低氧敏感性,建议鱼类SMR测定的流量设置不宜过快,并且SMR测定时间介于末次摄食后24~72h之间.
Oxygen consumption rate（MO2）is usually used to estimate the metabolism rate in fish energy metabolic studies.In order to investigate the effects of flow rate and measurement time on standard metabolic rate（SMR）in fishes,80 juvenile Qingbo（Spinibarbus sinensis）individuals with healthy body and similar body mass were used as fish model under the laboratory conditions of（25.0±0.5）℃.During the course of the experiment,five flow rates（1,2,3,4and 5L/h）were employed and each flow rate was continuously employed for six time points when SMR was measured per hour.The present study showed that differences in oxygen level（ΔMO2）of the outlets between the blank chamber and the experimental chamber decreased exponentially as the flow rate（v）increased,the equation being ΔMO2 =0.872 2v^（-0.999 8）（R^2=0.994,p〈0.001）.The ΔMO2 were affected significantly by the flow rate（F=20.994,p〈0.001）,with the maximum value appearing at 5L/h.The designed flow rates appeared to have no significant influence on SMR measurement of the juvenile S.sinensis（p〉0.05）.Additionally,the repeatability of SMR measured at different time points seemed to decline with increasing flow rate.Although there was no difference in average values of SMRamong the designed range of flow rate,the sample number,body mass,and fish＇s sensitivity to hypoxia should be considered before designing the energy metabolism experiment in fishes.The present study suggested that the flow rate for juvenile fish SMR measurement should not be too fast,and the measurement should be made between 24 and 72h after the last feeding.
作者
王蕾
付世建
曾令清
WANG Lei FU Shi-jian ZENG Ling-qing(Laboratory of Evolutionary Physiology and Behaviour, Chongqing Normal UniversitY, Chongqing 401331, China Chongqing Key Laboratory of Animal Biology, Chongqing 401331, China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第9期59-65,共7页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金(31300341)
重庆市自然科学基金(cstc2014jcyjA00018)
重庆市教委科技项目(KJ130619)
重庆师范大学青年人才拔尖培育计划项目(02030307-00027)
关键词
标准代谢率
流量
时间
稳定性
中华倒刺鲃
standard metabolic rate
flow rate
time
repeatability
Spinibarbus sinensis
