摘要
目的:研究过量人参皂苷对原代培养小鼠肝细胞的毒性作用。方法:小鼠原代肝细胞培养液中加入人参皂苷(终浓度分别为4000 mg/L,200 mg/L和10 mg/L),设为高、中、低浓度组,台盼蓝拒染法及MTT法测定人参皂苷对肝细胞增殖的影响,并测定肝细胞培养液中谷丙转氨酶(GPT)及谷草转氨酶(GOT)的含量。结果:与对照组比较,高浓度人参皂苷对原代培养肝细胞增殖有明显的抑制作用,给药后3 d、4 d、5 d、6 d和7 d,高浓度组活细胞数分别为对照组的85%、81%、71%、64%和59%。人参皂苷给药24 h开始,高浓度组培养液上清中GPT含量均显著升高(P<0.05);给药48 h、72 h和96 h时高浓度组细胞培养液GPT含量分别为对照组的2.6倍、8.0倍和12.5倍(P<0.01);随着给药时间延长,GPT含量呈上升趋势。给药72 h和96 h后高浓度组细胞与空白组比较,GOT含量均显著增加(P<0.05)。结论:过量人参皂苷能抑制肝细胞增殖,引起肝细胞损伤,对肝细胞可能存在直接的毒副作用。
Objective： To study the toxicity of excess Ginseng Saponin （GS） on primitive cultured mouse liver cells. Methods： GS was put into the primitive cultured mouse liver cells （final concentration respectively in 4000 mg/L, 200 mg/L and 10 mg/L）, which were set to high, medium and low concentration group. The effects of GS on the proliferation activity of liver cells in vitro were investigated by trypan blue experiments and MTT assay, while the content of glutamate pyruvic transaminase （GPT） and glutamic oxaloacetic transaminase （GOT） in liver cell cultures were detected. Results： Compared with blank control group, excessive GS had obvious inhibitory effect on the primitive cultured liver ceils proliferation. After delivery 3 d, 4 d, 5 d, 6 d and 7 d, living cells numbers of the high concentration group were respectively 85%, 81%, 71%, 64% and 59% of the control group. Given GS for 24 h, GPT levels of high concentration group culture were significantly elevated （P〈 0.05）, and GPT were respectively 2.6 times, 8.0 times and 12.5 times compared with the control group on 48 h, 72 h and 96 h （P〈0.01）. As drug prolonged, GPT contents were on the rise. Compared with the blank group, GOT contents of high concentration group were significantly increased on 72 h and 96 h （P〈0.05）. Conclusion： Excessive GS may have direct toxic effect on liver cells, by inhibiting hepatocyte proliferation and causing liver cell damage.
出处
《中医药导报》
2017年第18期33-36,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省教育厅科学研究项目(14C0125)
2015年度长沙医学院大学生研究性学习和创新性实验计划项目(37)
