MiRNA-373在皮肤鳞状细胞癌的表达及对侵袭的影响被引量：5

Expression of microRNA-373 in cutaneous squamous ceil＇carcinoma and its effect on cell invasion

导出

摘要目的分析microRNA-373（miR-373）在皮肤鳞状细胞癌（鳞癌）组织和细胞中的表达，探讨对皮肤鳞癌细胞侵袭的影响。方法实时 PCR检测皮肤鳞癌组织和细胞中miR-373的表达，将miR-373模拟物、miR-373抑制剂以及阴性对照miRNA转染皮肤鳞癌A431细胞，采用细胞侵袭实验分析miR-373表达下调对细胞侵袭的影响，采用Western 印迹分析miR-373表达下调对MMP-2和MMP-9蛋白表达的影响。结果皮肤鳞癌组织（2.465 ± 0.218）和SCL-1细胞及A431细胞（1.864 ± 0.178，2.919 ± 0.277）中miR-373的表达水平显著高于瘤旁组织和细胞（1.000 ± 0.000），差异有统计学意义（P 〈 0.05）。在转移性的皮肤鳞癌患者（3.323 ± 0.344）中，miR-373的表达显著高于非转移组（1.914 ± 0.161），差异有统计学意义（t = 4.158，P 〈 0.01）。与未处理组和阴性对照组相比，miR-373模拟物显著增加皮肤鳞癌A431细胞中miR-373的表达水平和细胞的侵袭能力，而miR-373的抑制剂显著下调皮肤鳞癌A431细胞中miR-373的表达水平和细胞的侵袭，差异有统计学意义（均P 〈 0.05）。结论 MiR-373表达下调显著抑制皮肤鳞癌A431细胞的侵袭，并能显著下调MMP-2和MMP-9蛋白的表达。 Objective To investigate the of microRNA-373 （miR-373） in cutaneous squamous cell carcinoma （CSCC） tissues and cells, and to explore its effects on cell invasion. Methods Real-time PCR was performed to determine the of miR-373 in CSCC tissues and paralesional normal skin tissues, as well as in CSCC cell lines （A431 and SCL-1） and HaCaT cells. A431 cells were divided into 4 groups： miR-373 mimic group, miR-373 inhibitor group and negative control group which were transfected with miR-373 mimic, miR-373 inhibitor and negative control miRNA respectively, and untreated group receiving no treatment. Cell invasion assay was performed to evaluate effects of miR-373 downregulation on cell invasion. Western blot analysis was conducted to assess effects of miR-373 downregulation on the protein of matrix metalloproteinase-2 （MMP-2） and MMP-9. Results Expression of miR-373 was significantly higher in the CSCC tissues（2.465 ± 0.218） than in the paralesional normal skin tissues （1.000 ± 0.000, P 〈 0.05）, and higher in SCL-1 cells （1.864 ± 0.178） and A431 cells（2.919 ± 0.277） than in HaCaT cells （1.000 ± 0.000, P 〈 0.05）. Most notably, miR-373 was also markedly higher in metastatic CSCC tissues than in non-metastatic CSCC tissues （3.323 ± 0.344 vs. 1.914 ± 0.161, t = 4.158, P = 0.000 4）. Compared with the untreated group and negative control group, the miR-373 mimic group showed significantly increased miR-373 and invasive ability, while the miR-373 inhibitor group showed markedly decreased miR-373 and invasive ability （all P 〈 0.05）. Conclusion MiR-373 downregulation can significantly suppress the invasion of A431 cells, and obviously decrease the protein of MMP-2 and MMP-9.