摘要
目的:对茄科Solanaceae植物甘肃武威香瓜茄Solanum muicatum成熟果实的化学成分进行分离纯化研究,并对分离得到的化合物进行化学成分鉴定。方法:采用95%的乙醇对香瓜茄干果进行加热提取,并依次用石油醚,乙酸乙酯,正丁醇进行萃取,然后采用薄层色谱法,正相硅胶柱色谱法,LH-20羟丙基葡聚糖凝胶色谱法和制备高效液相色谱法等方法对香瓜茄95%乙醇提取物的乙酸乙酯萃取部位进行分离纯化,并依据化合物的理化性质并通过核磁共振、质谱等现代波谱技术对化合物的化学结构进行鉴定。结果:最终从香瓜茄中分离纯化得到7个化合物,其中3个生物碱皂苷类化合物,2个黄酮类化合物及2个有机酸类化合物,分别鉴定为澳洲茄碱(solasonine 1),澳洲茄边碱(solamargine 2),澳洲茄胺(solasodine 3),槲皮素(quercetin 4),柚皮素(naringenin 5),阿魏酸(ferulic acid 6),熊果酸(ursone 7)。结论:以上7种化合物均为首次从该植物果实中分离纯化得到,该研究为综合开发及寻找天然植物性抗炎、抗肿瘤活性物质提供一定的化学依据和基础。
Objective： To study the chemical constituents from the mature fruits of Solarium muicatum Method： S. muicatumdry fruits were heated and extracted by using 95% ethanol, and then were extracted with petroleum ether, ethyl acetate and n-butanol in turn. Ethyl acetate extract fraction of S. muicatum 95% ethanol extract was isolated and purified by using chromatography on silica gel, Sephadex LH-20 column chromatography and preparative HPLC methods. The structures of the compounds were then identified by analysis of NMR and spectroscopic data by comparison with those reported on the literature. Result： Seven compounds were isolated from 95% ethanol andthey were identified as solasonine （1）, solamargine （2）, solasodine （3）, quercetin （4）,naringenin （5） , ferulic acid （6） , and ursone （7）. Conclusion： All of the compounds were obtained from this plant for the first time, providing certain chemical basis and foundation for the comprehensive development and the search for natural plant anti-inflammatory and antitumor active substances
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第19期100-104,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
上海市进一步加快中医药事业发展三年行动计划项目(ZY3-CCCX-3-3044)
关键词
香瓜茄
成熟果实
核磁共振
化学成分
Solanum muicatum
mature fruits
nuclear magnetic resonance
chemical constituents
