摘要
建立了聚乳酸-羟基乙酸-三亚甲基碳酸酯(PLGA-TMC)三元共聚物中丙交酯(DL-LA)、乙交酯(GA)、三亚甲基碳酸酯(TMC)三种单体残留测定的气相色谱法。采用Gs BP-5毛细管色谱柱(30.0 m×0.32 mm,0.25μm),氢离子火焰检测器(FID),进样口温度250℃,检测器温度300℃,柱温135℃,分流比为10:1。结果显示,DL-LA、GA和TMC分离良好,精密度试验RSD值均不大于2.0%,三种单体在相应范围内线性关系良好,相关系数均在0.999以上,平均加样回收率均在97.17%~101.71%,且RSD值均不大于5.0%。该方法专属性强、准确度高,可用于PLGA-TMC三元共聚物中DL-LA、GA、TMC三种单体的定量检测,更好地控制PLGA-TMC的质量。
To establish a method of determination of residual DL-lactide（DL-LA）,glycolide（GA） and 1,3-trimethylene carbonate（TMC） in DL-lactide-glycolide-trimethylene carbonate terpolymers（PLGA-TMC） by gas chromatography.Gs BP-5capillary column（30 m×0.32 mm,0.25 μm）,and FID detecter were used.The inlet temperature was set 250℃,the temperature of FID was 300℃,the column temperature was kept at 135℃,and the split ratio was 10：1.The results showed that DL-LA,GA and TMC were well separated,the relative standard deviations（RSDs） were all less than 2.0%,each monomer had a good linear relationship in the appropriate concentration range,and the correlation coefficient was not less than 0.999,the average recoveries were 97.17%~101.71%,and the RSDs were less than 5.0%.The method has good specificity and high accuracy,and can be used in the determination of residual monomers and the quality control of PLGA-TMC.
出处
《生物医学工程研究》
北大核心
2017年第3期268-270,276,共4页
Journal Of Biomedical Engineering Research
基金
山东省重点研发计划项目(2015GSF118089)
